Twentyman P R, Wright K A
MRC Clinical Oncology and Radiotherapeutics Unit, Cambridge, U.K.
Eur J Cancer. 1991;27(1):6-8. doi: 10.1016/0277-5379(91)90049-j.
The effects of human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) 1-1000 U/ml on the growth of human lung cancer cell lines have been studied in vitro. A panel of 10 small cell, 1 adenocarcinoma and 1 large cell lines was used with multidrug resistant sublines of 3 of the panel. The MTT assay was used to quantify cell numbers after 6-8 days' growth in the presence of GM-CSF. Neither growth inhibition nor stimulation of any of the cell lines in the presence of GM-CSF was observed. Any effects of this agent on residual tumour cells may not therefore present a problem during its clinical use to stimulate marrow regeneration after high-dose chemotherapy for lung cancer.
已在体外研究了人重组粒细胞-巨噬细胞集落刺激因子（GM-CSF）1-1000 U/ml 对人肺癌细胞系生长的影响。使用了一组 10 个小细胞、1 个腺癌和 1 个大细胞系，其中 3 个细胞系有耐多药亚系。采用 MTT 法在 GM-CSF 存在的情况下培养 6-8 天后对细胞数量进行定量。未观察到 GM-CSF 存在时对任何细胞系有生长抑制或刺激作用。因此，在肺癌大剂量化疗后使用该药物刺激骨髓再生的临床应用过程中，其对残留肿瘤细胞的任何影响可能不会构成问题。
