通过亲和电泳对胆碱酯酶进行结构和功能研究。
Structural and functional investigations of cholinesterases by means of affinity electrophoresis.
作者信息
Masson P
机构信息
Centre de Recherches du Service de Santé des Armées, Unité des Biochemie, La Tronche, France.
出版信息
Cell Mol Neurobiol. 1991 Feb;11(1):173-89. doi: 10.1007/BF00712808.
Abstract
- After a brief survey of the basic affinity electrophoresis concepts, the usual ways for preparing affinity electrophoresis ligands are examined. 2. Then results obtained on cholinesterases are reviewed. This section includes (a) structural and functional investigations on anionic sites, i.e., study of ligand-induced conformational change, organophosphate-induced "aging," genetic variants, and active-site topology; and (b) characterization of cholinesterase conjugates (hybrid proteins) and glycoinositol phospholipid-anchored cholinesterases. 3. The future prospects of affinity electrophoresis, e.g., investigations on the esteratic site and exploration of the carbohydrate moiety, are emphasized in the concluding section.
摘要
- 在简要概述了亲和电泳的基本概念之后，研究了制备亲和电泳配体的常用方法。2. 接着回顾了在胆碱酯酶方面取得的成果。本节包括：(a) 对阴离子位点的结构和功能研究，即配体诱导的构象变化、有机磷酸酯诱导的“老化”、遗传变异以及活性位点拓扑结构的研究；(b) 胆碱酯酶缀合物（杂合蛋白）和糖基磷脂酰肌醇锚定胆碱酯酶的表征。3. 在结论部分强调了亲和电泳的未来前景，例如对酯解位点的研究和对碳水化合物部分的探索。
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