Honoré B, Rasmussen H H, Vandekerckhove J, Celis J E
Institute of Medical Biochemistry, Aarhus University, Denmark.
FEBS Lett. 1991 Mar 25;280(2):235-40. doi: 10.1016/0014-5793(91)80300-r.
Neuronal protein gene product 9.5 (PGP 9.5) most likely identical to ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) has been reported to be expressed almost exclusively in neuronal and neuroendocrine tissues. By two-dimensional (2D) immunoblotting, comigration and microsequencing of proteins recovered from 2D gels we have identified PGP 9.5 UCH-L1 as polypeptide IEF SSP 6104 (Mr = 27,000, pI = 5.49) in the comprehensive 2D gel cellular protein database of human embryonal lung MRC-5 fibroblasts [(1989) Electrophoresis 10, 76 115; (1990) Electrophoresis 11, 1072 1113]. This protein is expressed at high levels in quiescent and proliferating cultured normal fibroblasts and is strongly down-regulated (about 10 times) in their transformed counterparts.
据报道，神经元蛋白基因产物9.5（PGP 9.5）很可能与泛素羧基末端水解酶同工酶L1（UCH-L1）相同，几乎只在神经元和神经内分泌组织中表达。通过二维（2D）免疫印迹、从2D凝胶中回收的蛋白质的共迁移和微测序，我们在人胚肺MRC-5成纤维细胞的综合2D凝胶细胞蛋白质数据库中，将PGP 9.5 UCH-L1鉴定为多肽IEF SSP 6104（Mr = 27,000，pI = 5.49）[（1989年）《电泳》10, 76 - 115；（1990年）《电泳》11, 1072 - 1113]。这种蛋白质在静止和增殖的培养正常成纤维细胞中高水平表达，而在其转化的对应细胞中则强烈下调（约10倍）。
