利用碳水化合物玻璃保护、构建图案并搭建支撑脂质膜

Protecting, patterning, and scaffolding supported lipid membranes using carbohydrate glasses.

作者信息

Oliver Ann E, Kendall Eric L, Howland Michael C, Sanii Babak, Shreve Andrew P, Parikh Atul N

机构信息

Department of Applied Science, University of California-Davis, 1 Shields Ave., Davis, CA 95616, USA.

出版信息

Lab Chip. 2008 Jun;8(6):892-7. doi: 10.1039/b800370j. Epub 2008 Apr 11.

DOI:10.1039/b800370j

PMID:18497908

Abstract

Disaccharides are known to protect sensitive biomolecules against stresses caused by dehydration, both in vivo and in vitro. Here we demonstrate how interfacial accumulation of trehalose can be used to (1) produce rugged supported lipid bilayers capable of near total dehydration; (2) enable spatial patterning of membrane micro-arrays; and (3) form stable bilayers on otherwise lipophobic substrates (e.g., metal transducers) thus affording protecting, patterning, and scaffolding of lipid bilayers.

摘要

已知双糖可在体内和体外保护敏感生物分子免受脱水引起的应激。在此，我们展示了海藻糖的界面积累如何能够用于：（1）制备能够近乎完全脱水的坚固支撑脂质双层；（2）实现膜微阵列的空间图案化；以及（3）在原本疏脂的底物（如金属换能器）上形成稳定的双层，从而为脂质双层提供保护、图案化和支架作用。