Centre for Nanoscience and Fakultat fur Physik, Ludwig-Maximilians-Universitat, Geschwister-Scholl-Platz 1, Munchen D-80539, Germany.
Biointerphases. 2010 Mar;5(1):1-8. doi: 10.1116/1.3319326.
Controlled deposition of lipid bilayers plays a key role in creating supported membranes for biosensing devices and biophysical cell studies. The authors adopt a solvent-exchange method in order to deposit a phospholipid bilayer on solid substrates. The basic concept of deposition is to dissolve phospholipids in isopropanol-water mixtures and to increase water content gradually. Shortly before the onset of the micelle-to-vesicle transition, a lipid bilayer nucleates at the solid surface. They investigate the bulk phase behavior and surface coverage using small angle x-ray scattering and attenuated total reflection-Fourier transform infrared spectroscopy. They find a sequence of transitions from inverted-monomeric-micellar and vesicle phases correlating with an increasing amount of lipid on the adsorption layer. Supported lipid bilayers, prepared using this approach, are homogeneous and fluid.
控制脂质双层的沉积在为生物传感设备和生物物理细胞研究创建支撑膜方面起着关键作用。作者采用溶剂交换方法在固体基底上沉积磷脂双层。沉积的基本概念是将磷脂溶解在异丙醇-水混合物中，并逐渐增加水的含量。在胶束到囊泡转变开始之前不久，脂质双层在固体表面成核。他们使用小角 X 射线散射和衰减全反射傅里叶变换红外光谱法研究了体相行为和表面覆盖率。他们发现从反相单体胶束和囊泡相的一系列转变与吸附层上脂质量的增加相关。使用这种方法制备的支撑脂质双层是均匀的和流动的。
