Szentmáry Nóra, Szende Béla, Süveges Ildikó
Department of Ophthalmology, Semmelweis University, Budapest, Hungary.
Histol Histopathol. 2008 Aug;23(8):911-6. doi: 10.14670/HH-23.911.
Our purpose was to elucidate the pathways of apoptosis of corneas with Fuchs' dystrophy and pseudophakic bullous keratopathy. Sixteen corneal buttons (14 patients, median age 73 years) with Fuchs' dystrophy, 13 with pseudophakic bullous keratopathy (PBK) (13 patients, median age 69 years) and 8 buttons (8 patients, median age 59 years) from enucleated eyes with chorioideal melanoma (controls) were analysed histologically. Immunohistochemical analysis was performed to investigate the expression of p21, p27, p63, survivin, CD95, cathepsin, bax, bcl-2 and Ki67. Positive immunohistochemical reactions were detected in epithelial cells of the corneas, but keratocytes and endothelial cells were not positive in any of the groups or stainings. The number of p27 and survivin positive epithelial cells was significantly lower (p=0.048 and 0.041) and the number of cathepsin positive epithelial cells was significantly higher (p=0.004) in Fuchs' dystrophy corneas compared to controls. In pseudophakic bullous keratopathy, p21 and p27 positive epithelial cells were present in a significantly lower (p=0.02 and 0.005) number than in controls. We conclude that genetically programmed cell death is related to the p27, cathepsin and survivin pathways in Fuchs' dystrophy and to the p21 and p27 pathways in pseudophakic bullous keratopathy.
我们的目的是阐明富克斯角膜营养不良和假晶状体大疱性角膜病变中角膜细胞凋亡的途径。对16个富克斯角膜营养不良的角膜组织块（14例患者，中位年龄73岁）、13个假晶状体大疱性角膜病变（PBK）的角膜组织块（13例患者，中位年龄69岁）以及8个来自脉络膜黑色素瘤摘除眼球的角膜组织块（8例患者，中位年龄59岁）（作为对照）进行了组织学分析。进行免疫组织化学分析以研究p21、p27、p63、存活素、CD95、组织蛋白酶、bax、bcl - 2和Ki67的表达。在角膜上皮细胞中检测到阳性免疫组织化学反应，但在任何组或染色中角膜基质细胞和内皮细胞均为阴性。与对照组相比，富克斯角膜营养不良角膜中p27和存活素阳性上皮细胞数量显著减少（p = 0.048和0.041），而组织蛋白酶阳性上皮细胞数量显著增加（p = 0.004）。在假晶状体大疱性角膜病变中，p21和p27阳性上皮细胞数量比对照组显著减少（p = 0.02和0.005）。我们得出结论，程序性细胞死亡与富克斯角膜营养不良中的p27、组织蛋白酶和存活素途径以及假晶状体大疱性角膜病变中的p21和p27途径有关。
