Bifani Pablo, Mathema Barun, Kurepina Natalia, Shashkina Elena, Bertout Julie, Blanchis Anne Sophie, Moghazeh Soraya, Driscoll Jeffrey, Gicquel Brigitte, Frothingham Richard, Kreiswirth Barry N
Pasteur Institute, Brussels, Belgium.
J Infect Dis. 2008 Jul 1;198(1):90-4. doi: 10.1086/588822.
We describe the genotypic and phenotypic characteristics of a mono-rifampin-resistant (RIF(R)) Mycobacterium tuberculosis strain cluster (designated AU-RIF(R)) and the acquisition of additional drug resistance. Drug susceptibility, sequences of regions that determine drug resistance, and basic clinical data were examined. A rare codon duplication (514(TTC)) in rpoB conferring high levels of RIF(R) (minimum inhibitory concentration of >256 microg/mL) in 29 isolates was identified. AU-RIF(R) strains developed secondary resistance to isoniazid and 7 resistance combinations to 6 different antibiotics. Patients infected with AU-RIF(R) strains were primarily immunocompromised. These data suggest that host factors, such as HIV status, may allow dissemination of mono-RIF(R) strains and facilitate the accumulation of additional drug resistance.
我们描述了一株耐利福平（RIF(R)）结核分枝杆菌菌株簇（命名为AU-RIF(R)）的基因型和表型特征以及额外耐药性的获得情况。检测了药物敏感性、决定耐药性区域的序列以及基本临床数据。在29株分离菌株中，发现rpoB基因存在一种罕见的密码子重复（514(TTC)），导致高水平耐利福平（最低抑菌浓度>256μg/mL）。AU-RIF(R)菌株对异烟肼产生了继发性耐药，并对6种不同抗生素形成了7种耐药组合。感染AU-RIF(R)菌株的患者主要存在免疫功能低下的情况。这些数据表明，宿主因素，如HIV感染状态，可能会使耐单利福平菌株得以传播，并促进额外耐药性的积累。
