Tupinambá G, da Silva A J R, Alviano C S, Souto-Padron T, Seldin L, Alviano D S
Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
J Appl Microbiol. 2008 Oct;105(4):1044-53. doi: 10.1111/j.1365-2672.2008.03844.x. Epub 2008 May 21.
The purpose of this study was to investigate the antagonistic activity of Paenibacillus polymyxa strain SCE2 against mycotoxigenic fungi and to characterize the antimicrobial compound.
Strain SCE2 showed a broad inhibition spectrum against different mycotoxigenic fungi. The crude supernatant obtained from strain SCE2 was filtered with Amicon Diaflo membranes, and the antimicrobial activity was detected in the fraction ranging from 0.5 to 1 kDa. The bioautography of this fraction presented two inhibition zones with both indicator strains (Micrococcus sp. and Aspergillus versicolor), suggesting that more than one substance is produced by SCE2. Based on UV-visible spectral and liquid chromatography/mass spectrometry data, phenazine-1-carboxylic acid (PCA) was characterized as the major compound present in the highest purity active fraction. Drastic alterations in the cytoplasm of A. versicolor were observed by electron microscopy.
One of the antimicrobial substances produced by P. polymyxa SCE2 is PCA.
The broad antifungal spectrum observed by the compound produced by SCE2 suggests that it has the potential to be used as an alternative or supplementary method to chemical pesticides against mycotoxigenic fungi. This is the first description of a phenazine produced by a member of the genus Paenibacillus.
本研究旨在探究多粘芽孢杆菌SCE2菌株对产毒真菌的拮抗活性，并对其抗菌化合物进行表征。
SCE2菌株对不同的产毒真菌表现出广泛的抑制谱。用Amicon Diaflo膜过滤从SCE2菌株获得的粗提上清液，并在0.5至1 kDa的级分中检测到抗菌活性。该级分的生物自显影图谱对两种指示菌株（微球菌属和杂色曲霉）均呈现出两个抑制区，表明SCE2产生了不止一种物质。基于紫外可见光谱和液相色谱/质谱数据，吩嗪-1-羧酸（PCA）被表征为最高纯度活性级分中存在的主要化合物。通过电子显微镜观察到杂色曲霉细胞质发生了剧烈变化。
多粘芽孢杆菌SCE2产生的抗菌物质之一是PCA。
SCE2产生的化合物所观察到的广泛抗真菌谱表明，它有潜力作为一种替代或补充化学农药防治产毒真菌的方法。这是首次对芽孢杆菌属成员产生的吩嗪进行描述。
