Fosgate G T, Tavornpanich S, Hunter D, Pugh R, Sterle J A, Schumann K R, Eberling A J, Beckham T R, Martin B M, Clarke N P, Adams L G
Departments of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, USA.
Vet Microbiol. 2008 Nov 25;132(1-2):158-64. doi: 10.1016/j.vetmic.2008.04.016. Epub 2008 May 21.
Foot-and-mouth disease virus (FMDV) and classical swine fever virus (CSFV) are highly contagious and can cause great economic losses when introduced into disease-free regions. Accurate estimates of diagnostic specificity (Sp) are important when considering the implementation of surveillance for these agents. The purpose of this study was to estimate diagnostic Sp of a real-time reverse-transcriptase PCR assay developed for detection of FMDV in cattle and domestic swine and CSFV in domestic swine based on non-invasive specimen collection. One thousand and eighty-eight range beef cattle were sampled from thirteen geographic locations throughout Texas. One thousand and one hundred market hogs and cull sows were sampled. Results for both FMDV and CSFV were considered positive if amplification occurred at or before 40 PCR cycles, inconclusive between 40 and 45 cycles and negative otherwise. Ten cattle had nonspecific PCR amplifications for FMDV, but none were classified as positive and only one as inconclusive. Specificity (95% confidence interval) was estimated as 100% (99.7, 100). There were 19 nonspecific PCR amplifications for FMDV in sampled swine with 1 classified as positive, 6 as inconclusive, and 12 as negative. Specificity (95% confidence interval) was estimated as 99.9% (99.5, 100). There were 21 nonspecific PCR amplifications for CSFV, and 1 was classified as positive. Specificity (95% confidence interval) was estimated as 99.9% (99.5, 100). These assays have high Sp, but nonspecific PCR amplifications can occur.
口蹄疫病毒（FMDV）和猪瘟病毒（CSFV）具有高度传染性，一旦传入无病地区，可能会造成巨大经济损失。在考虑对这些病原体实施监测时，准确估计诊断特异性（Sp）非常重要。本研究的目的是基于非侵入性样本采集，估计一种为检测牛和家猪中的FMDV以及家猪中的CSFV而开发的实时逆转录聚合酶链反应（RT-PCR）检测方法的诊断Sp。从德克萨斯州的13个地理位置采集了1088头育肥牛样本。还采集了1100头商品猪和淘汰母猪的样本。如果在40个PCR循环或之前出现扩增，则FMDV和CSFV的结果均被视为阳性；在40至45个循环之间为不确定；否则为阴性。10头牛出现了FMDV的非特异性PCR扩增，但没有一头被分类为阳性，只有一头为不确定。特异性（95%置信区间）估计为100%（99.7，100）。在采集的猪样本中，有19次FMDV的非特异性PCR扩增，其中1次被分类为阳性，6次为不确定，12次为阴性。特异性（95%置信区间）估计为99.9%（99.5，100）。有21次CSFV的非特异性PCR扩增，其中1次被分类为阳性。特异性（95%置信区间）估计为99.9%（99.5，100）。这些检测方法具有较高的Sp，但可能会出现非特异性PCR扩增。
