Shen Lihua, Li Xiaoxia, Qi Honglan, Zhang Chengxiao
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
Luminescence. 2008 Nov-Dec;23(6):370-5. doi: 10.1002/bio.1046.
Electrogenerated chemiluminescence (ECL) of a ruthenium complex polymer modified carbon paste electrode and its analytical applications were investigated. The ruthenium complex polymer was prepared using bis(2,2-bipyridine) (4,4-dicarboxy-2,2-bipyridine) ruthenium(II). The ECL behaviours of ruthenium complex polymer modified carbon paste electrode were investigated in the absence and presence of tripropylamine (TPA). The modified carbon paste electrode exhibited long-term stability and fine reproducibility. The ECL intensity of the modified carbon paste electrode was linear with the concentration of TPA in the range 2.0 x 10(-6)-3.8 x 10(-3) mol/L, with a detection limit (S:N = 3) of 6 x 10(-7) mol/L. It was also found that raceanisodamine could enhance the ECL intensity of the modified electrode. The ECL intensity of the modified carbon paste electrode was linear with the concentration of raceanisodamine in the range 1.1 x 10(-5)-6.0 x 10(-4) mol/L, with a detection limit (S:N = 3) of 6 x 10(-6) mol/L. This work demonstrates that the entrapment of ruthenium complex in a highly cross-linked polymer is a promising approach to construct an ECL modified electrode with long-term stability and fine reproducibility. The modified electrode designed has a potential application in the ECL detector.
研究了钌配合物聚合物修饰碳糊电极的电致化学发光（ECL）及其分析应用。使用双（2,2-联吡啶）（4,4-二羧基-2,2-联吡啶）钌（II）制备钌配合物聚合物。在不存在和存在三丙胺（TPA）的情况下研究了钌配合物聚合物修饰碳糊电极的ECL行为。修饰后的碳糊电极表现出长期稳定性和良好的重现性。修饰碳糊电极的ECL强度与TPA浓度在2.0×10⁻⁶ - 3.8×10⁻³ mol/L范围内呈线性关系，检测限（S:N = 3）为6×10⁻⁷ mol/L。还发现消旋山莨菪碱可以增强修饰电极的ECL强度。修饰碳糊电极的ECL强度与消旋山莨菪碱浓度在1.1×10⁻⁵ - 6.0×10⁻⁴ mol/L范围内呈线性关系，检测限（S:N = 3）为6×10⁻⁶ mol/L。这项工作表明，将钌配合物包埋在高度交联的聚合物中是构建具有长期稳定性和良好重现性的ECL修饰电极的一种有前途的方法。所设计的修饰电极在ECL检测器中具有潜在的应用。
