Zhang Meng-Lin, Sheng Guo-Ping, Yu Han-Qing
Department of Chemistry, University of Science & Technology of China, Hefei, China.
Water Res. 2008 Jul;42(13):3464-72. doi: 10.1016/j.watres.2008.04.010. Epub 2008 Apr 15.
A simple and sensitive method was developed for the determination of low-concentration proteins and carbohydrates in the effluents from biological wastewater treatment reactors using resonance light-scattering (RLS) technique. Two ionic dyes, Congo red and Neutral red were, respectively used as an RLS probes for the determination of proteins and carbohydrates. This method is based on the interactions between biomacromolecules and dyes, which cause a substantial increase in the resonance scattering signal of dyes in the wavelength range of 200-650 nm. The characteristics of RLS spectra of the macromolecule-dye complexes, influencing factors, and optimum analytical conditions for the measurement were explored. The method was satisfactorily applied to the measurement of proteins and carbohydrates in the effluents from 10 aerobic or anaerobic bioreactors, and a high sensitivity were achieved.
