使用改良注射器针头对大鼠眶下神经进行慢性压迫损伤
Chronic constriction injury of the infraorbital nerve in the rat using modified syringe needle.
作者信息
Kernisant Melanie, Gear Robert W, Jasmin Luc, Vit Jean-Philippe, Ohara Peter T
机构信息
Department of Anatomy, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, United States.
出版信息
J Neurosci Methods. 2008 Jul 15;172(1):43-7. doi: 10.1016/j.jneumeth.2008.04.013. Epub 2008 Apr 22.
Abstract
Here we report a method for performing a chronic constriction injury (CCI) of the infraorbital nerve (ION) in the rat as a component of a chronic pain model. The surgical approach to the ION is described together with the use of a modified dental syringe needle that simplifies placing two chromic gut ligatures around the ION. This method makes the surgical procedure easier, the nerve injury more consistent across animals and reduces secondary damage to the ION and surrounding tissue. Pain behavior testing together with immunostaining for markers of nerve injury in the spinal trigeminal nucleus show the suitability of this procedure as a model of orofacial pain.
摘要
在此，我们报告一种在大鼠中对眶下神经（ION）进行慢性压迫损伤（CCI）的方法，作为慢性疼痛模型的一部分。描述了对ION的手术入路，以及使用改良牙科注射器针头，该针头简化了在ION周围放置两个铬肠线结扎的操作。这种方法使手术过程更简便，不同动物之间的神经损伤更一致，并减少了对ION和周围组织的继发性损伤。疼痛行为测试以及对三叉神经脊束核中神经损伤标志物的免疫染色表明，该手术作为口面部疼痛模型是合适的。
相似文献
1
Chronic constriction injury of the infraorbital nerve in the rat using modified syringe needle.使用改良注射器针头对大鼠眶下神经进行慢性压迫损伤
J Neurosci Methods. 2008 Jul 15;172(1):43-7. doi: 10.1016/j.jneumeth.2008.04.013. Epub 2008 Apr 22.
2
Behavioral evidence of trigeminal neuropathic pain following chronic constriction injury to the rat's infraorbital nerve.大鼠眶下神经慢性压迫损伤后三叉神经病理性疼痛的行为学证据。
J Neurosci. 1994 May;14(5 Pt 1):2708-23. doi: 10.1523/JNEUROSCI.14-05-02708.1994.
3
Behavioral study of non-evoked orofacial pain following different types of infraorbital nerve injury in rats.大鼠不同类型眶下神经损伤后非诱发性口面部疼痛的行为学研究
Physiol Behav. 2015 Jan;138:292-6. doi: 10.1016/j.physbeh.2014.10.009. Epub 2014 Oct 24.
4
An Improved Rodent Model of Trigeminal Neuropathic Pain by Unilateral Chronic Constriction Injury of Distal Infraorbital Nerve.通过单侧眶下神经远端慢性压迫损伤建立的改良三叉神经病理性疼痛啮齿动物模型
J Pain. 2017 Aug;18(8):899-907. doi: 10.1016/j.jpain.2017.02.427. Epub 2017 Feb 24.
5
Chronic Constriction Injury of the Distal Infraorbital Nerve (DIoN-CCI) in Mice to Study Trigeminal Neuropathic Pain.建立小鼠眶下神经慢性缩窄性损伤（DIoN-CCI）模型用于研究三叉神经病理性疼痛
J Vis Exp. 2024 Mar 8(205). doi: 10.3791/66420.
6
Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain.大鼠眶下神经慢性压迫损伤（IoN-CCI）用于研究三叉神经病理性疼痛
J Vis Exp. 2015 Sep 21(103):53167. doi: 10.3791/53167.
7
Pain threshold monitoring during chronic constriction injury of the infraorbital nerve in rats.大鼠眶下神经慢性压迫损伤期间的疼痛阈值监测
Br J Neurosurg. 2019 Aug;33(4):409-412. doi: 10.1080/02688697.2018.1538483. Epub 2018 Nov 15.
8
Differential effects of calcitonin gene-related peptide receptor blockade by olcegepant on mechanical allodynia induced by ligation of the infraorbital nerve vs the sciatic nerve in the rat.降钙素基因相关肽受体拮抗剂 olcegepant 对眶下神经结扎和坐骨神经结扎诱导的大鼠机械性痛觉过敏的差异作用。
Pain. 2012 Sep;153(9):1939-1948. doi: 10.1016/j.pain.2012.06.009. Epub 2012 Jul 15.
9
Nonevoked facial pain in rats following infraorbital nerve injury: a parametric analysis.眶下神经损伤后大鼠的非诱发性面部疼痛：参数分析
Physiol Behav. 2004 Jun;81(4):595-604. doi: 10.1016/j.physbeh.2004.02.026.
10
Involvement of astroglial glutamate-glutamine shuttle in modulation of the jaw-opening reflex following infraorbital nerve injury.眶下神经损伤后，星形胶质细胞谷氨酸-谷氨酰胺穿梭在调节张口反射中的作用。
Eur J Neurosci. 2014 Jun;39(12):2050-9. doi: 10.1111/ejn.12562. Epub 2014 Mar 25.
引用本文的文献
1
A glutamatergic brain neural circuit is critical for modulating trigeminal neuropathic pain.谷氨酸能脑神经网络回路对调节三叉神经病理性疼痛至关重要。
Pain. 2025 May 1. doi: 10.1097/j.pain.0000000000003647.
2
Potent painkiller from spider venom antagonizes P2X3 receptors without dysgeusia.源自蜘蛛毒液的强效止痛药可拮抗P2X3受体且无味觉障碍。
Mol Ther. 2025 Feb 5;33(2):771-785. doi: 10.1016/j.ymthe.2024.12.036. Epub 2024 Dec 31.
3
Trigeminal neuralgia.三叉神经痛
Nat Rev Dis Primers. 2024 May 30;10(1):39. doi: 10.1038/s41572-024-00523-z.
4
LncRNA Anxa10-203 enhances Mc1r mRNA stability to promote neuropathic pain by recruiting DHX30 in the trigeminal ganglion.长链非编码RNA Anxa10-203通过在三叉神经节中招募DHX30来增强Mc1r信使核糖核酸稳定性，从而促进神经性疼痛。
J Headache Pain. 2024 Mar 4;25(1):28. doi: 10.1186/s10194-024-01733-2.
5
Transcriptome-wide analysis of trigeminal ganglion and subnucleus caudalis in a mouse model of chronic constriction injury-induced trigeminal neuralgia.慢性缩窄性损伤诱导的三叉神经痛小鼠模型中三叉神经节和尾侧亚核的全转录组分析
Front Pharmacol. 2023 Sep 28;14:1230633. doi: 10.3389/fphar.2023.1230633. eCollection 2023.
6
GFAP-NpHR mediated optogenetic inhibition of trigeminal nucleus caudalis attenuates hypersensitive behaviors and thalamic discharge attributed to infraorbital nerve constriction injury.GFAP-NpHR 介导的三叉神经尾核光遗传抑制减轻眶下神经缩窄损伤所致的超敏行为和丘脑放电。
J Headache Pain. 2023 Oct 11;24(1):137. doi: 10.1186/s10194-023-01669-z.
7
Chemokine CCL7 mediates trigeminal neuropathic pain CCR2/CCR3-ERK pathway in the trigeminal ganglion of mice.趋化因子 CCL7 通过 CCR2/CCR3-ERK 通路在小鼠三叉神经节中介导神经病理性疼痛。
Mol Pain. 2023 Jan-Dec;19:17448069231169373. doi: 10.1177/17448069231169373.
8
Accumulation of RIPK1 into mitochondria is requisite for oxidative stress-mediated necroptosis and proliferation in Rat Schwann cells.RIPK1 在 线 粒体 中的 积累 是 氧化 应激 介 导 的 许旺 细胞 坏死 和 增殖 的 必要 条件。
Int J Med Sci. 2022 Oct 31;19(13):1965-1976. doi: 10.7150/ijms.69992. eCollection 2022.
9
MiR-223-3p alleviates trigeminal neuropathic pain in the male mouse by targeting MKNK2 and MAPK/ERK signaling.miR-223-3p 通过靶向 MKNK2 和 MAPK/ERK 信号通路缓解雄性小鼠三叉神经病理性疼痛。
Brain Behav. 2022 Jul;12(7):e2634. doi: 10.1002/brb3.2634. Epub 2022 May 24.
10
Pain Relief in a Trigeminal Neuralgia Model Optogenetic Inhibition on Trigeminal Ganglion Itself With Flexible Optic Fiber Cannula.三叉神经痛模型中的疼痛缓解：使用柔性光纤套管对三叉神经节进行光遗传学抑制。
Front Cell Neurosci. 2022 Apr 28;16:880369. doi: 10.3389/fncel.2022.880369. eCollection 2022.
本文引用的文献
1
Silencing the Kir4.1 potassium channel subunit in satellite glial cells of the rat trigeminal ganglion results in pain-like behavior in the absence of nerve injury.沉默大鼠三叉神经节卫星神经胶质细胞中的Kir4.1钾通道亚基会在没有神经损伤的情况下导致疼痛样行为。
J Neurosci. 2008 Apr 16;28(16):4161-71. doi: 10.1523/JNEUROSCI.5053-07.2008.
2
Sodium channel Nav1.6 accumulates at the site of infraorbital nerve injury.钠通道Nav1.6在眶下神经损伤部位聚集。
BMC Neurosci. 2007 Jul 27;8:56. doi: 10.1186/1471-2202-8-56.
3
Intracisternal administration of mitogen-activated protein kinase inhibitors reduced mechanical allodynia following chronic constriction injury of infraorbital nerve in rats.在大鼠眶下神经慢性压迫损伤后，脑池内给予丝裂原活化蛋白激酶抑制剂可减轻机械性异常性疼痛。
Prog Neuropsychopharmacol Biol Psychiatry. 2007 Aug 15;31(6):1322-9. doi: 10.1016/j.pnpbp.2007.05.016. Epub 2007 Jun 7.
4
Mechanisms involved in modulation of trigeminal primary afferent activity in rats with peripheral mononeuropathy.外周单神经病大鼠三叉神经初级传入活动调节的相关机制
Eur J Neurosci. 2006 Oct;24(7):1976-86. doi: 10.1111/j.1460-9568.2006.05065.x. Epub 2006 Oct 16.
5
Efficacy of propentofylline, a glial modulating agent, on existing mechanical allodynia following peripheral nerve injury.神经胶质调节剂丙戊茶碱对外周神经损伤后现有的机械性异常性疼痛的疗效。
Brain Behav Immun. 2007 Feb;21(2):238-46. doi: 10.1016/j.bbi.2006.07.001. Epub 2006 Sep 1.
6
Autonomic fibre sprouting and changes in nociceptive sensory innervation in the rat lower lip skin following chronic constriction injury.大鼠下唇皮肤慢性缩窄性损伤后自主神经纤维芽生及伤害性感觉神经支配的变化
Eur J Neurosci. 2005 May;21(9):2475-87. doi: 10.1111/j.1460-9568.2005.04089.x.
7
Trigeminal neuralgia: current treatments and future developments.三叉神经痛：当前治疗方法与未来发展
Expert Opin Emerg Drugs. 2003 May;8(1):123-43. doi: 10.1517/14728214.8.1.123.
8
A simple method for reconditioning epoxy-coated microelectrodes for extracellular single neuron recording.一种用于细胞外单神经元记录的环氧涂层微电极修复的简单方法。
J Neurosci Methods. 2003 Mar 15;123(2):215-7. doi: 10.1016/s0165-0270(02)00365-5.
9
Contribution of injured and uninjured dorsal root ganglion neurons to pain behavior and the changes in gene expression following chronic constriction injury of the sciatic nerve in rats.大鼠坐骨神经慢性缩窄损伤后，受损和未受损背根神经节神经元对疼痛行为的作用及基因表达变化
Pain. 2003 Jan;101(1-2):65-77. doi: 10.1016/s0304-3959(02)00296-8.
10
Experimental model of trigeminal pain in the rat by constriction of one infraorbital nerve: changes in neuronal activities in the somatosensory cortices corresponding to the infraorbital nerve.通过结扎一侧眶下神经建立大鼠三叉神经痛实验模型：与眶下神经对应的体感皮层神经元活动的变化
Exp Brain Res. 1999 Jun;126(3):383-98. doi: 10.1007/s002210050745.
Zotero 插件