Mazzucotelli Anne, Ribet Carole, Castan-Laurell Isabelle, Daviaud Danièle, Guigné Charlotte, Langin Dominique, Valet Philippe
Institut National de la Santé et de la Recherche Médicale, U858, Toulouse, France.
Regul Pept. 2008 Oct 9;150(1-3):33-7. doi: 10.1016/j.regpep.2008.04.003. Epub 2008 Apr 13.
By using pangenomic microarray, we identified apelin as a unique adipokine up regulated by the transcriptional co-activator peroxisome proliferator-activated receptor gamma (PPARgamma) co-activator 1alpha (PGC-1alpha) in human white adipocytes. We investigated its regulation in vitro and in vivo. Overexpression of PGC-1alpha by adenovirus in human adipocytes induces apelin expression and secretion. Pharmacological induction of cAMP, an upstream regulator of endogenous PGC-1alpha expression, up regulates apelin gene expression and also apelin secretion in human and mice adipocytes. Moreover, during cold exposure in mice, a physiological situation known to induce both cAMP and PGC-1alpha, apelin expression in adipocytes and plasma levels were increased. This is the first demonstration that PGC-1alpha is involved in the regulation of an adipokine gene expression and release.
通过使用泛基因组微阵列，我们确定阿片肽是一种独特的脂肪因子，在人白色脂肪细胞中由转录共激活因子过氧化物酶体增殖物激活受体γ（PPARγ）共激活因子1α（PGC-1α）上调。我们在体外和体内研究了其调节作用。通过腺病毒在人脂肪细胞中过表达PGC-1α可诱导阿片肽的表达和分泌。内源性PGC-1α表达的上游调节因子cAMP的药理学诱导可上调人及小鼠脂肪细胞中阿片肽基因的表达以及阿片肽的分泌。此外，在小鼠冷暴露期间，已知这种生理状况会诱导cAMP和PGC-1α，脂肪细胞中的阿片肽表达和血浆水平会升高。这是首次证明PGC-1α参与脂肪因子基因表达和释放的调节。
