Hagren Virve, von Lode Piia, Syrjälä Anniina, Korpimäki Teemu, Tuomola Mika, Kauko Otto, Nurmi Jussi
Abacus Diagnostica Ltd., Tykistökatu 4D, FI-20520 Turku, Finland.
Int J Food Microbiol. 2008 Jul 15;125(2):158-61. doi: 10.1016/j.ijfoodmicro.2008.03.037. Epub 2008 Apr 4.
We describe a system consisting of rapid sample enrichment and homogeneous end-point PCR analysis that enables the detection of Salmonella in various food matrices in 8 h. Sample preparation starts with 6 h enrichment step in supplemented broth, after which Salmonella cells are collected with immunomagnetic particles. The particles are washed and dispensed to ready-to-use PCR reaction vessels, which contain dried assay-specific reagents and an internal amplification control. PCR is performed with a novel instrument platform utilising the sensitive label technology of time-resolved fluorometry. Qualitative assay results are automatically interpreted and available in 45 min after sample addition. The overall accuracy, sensitivity and specificity of the Magda CA Salmonella system were 99.1%, 98.4% and 100.0%, respectively, based on the evaluation of 107 samples (beef, pork, poultry and ready-to-eat meals) artificially contaminated with sub-lethally injured Salmonella cells.
我们描述了一种由快速样品富集和均相终点PCR分析组成的系统，该系统能够在8小时内检测各种食品基质中的沙门氏菌。样品制备首先在补充肉汤中进行6小时的富集步骤，之后用免疫磁珠收集沙门氏菌细胞。磁珠经过洗涤后被分配到即用型PCR反应管中，反应管中含有干燥的特定检测试剂和内部扩增对照。PCR使用一种利用时间分辨荧光测定法的灵敏标记技术的新型仪器平台进行。定性检测结果会自动解读，在加入样品后45分钟即可获得。基于对107份人工污染了亚致死损伤沙门氏菌细胞的样品（牛肉、猪肉、家禽和即食餐）的评估，Magda CA沙门氏菌系统的总体准确度、灵敏度和特异性分别为99.1%、98.4%和100.0%。
