Plank Johann, Andres Philip R, Krause Ingolf, Winter Christian
Chair for Construction Chemicals, Department of Chemistry, Technische Universität München, Lichtenbergstr. 4, 85747 Garching, Germany.
Protein Expr Purif. 2008 Aug;60(2):176-81. doi: 10.1016/j.pep.2008.04.003. Epub 2008 Apr 20.
Casein is used as an additive in binders or paints and as such exhibits unique properties which might be based on the properties of certain subproteins in the complex whole casein mixture. Therefore, the separation of whole casein (CN) from cow milk was performed on a gram scale in order to yield sufficient amounts of the protein subfractions alpha-, beta-, and kappa-casein for further testing utilizing fast protein liquid chromatography (FPLC) and preceding enrichment in the case of kappa-casein. Construction chemical grade casein, which differs in quality from dairy grade casein, was used for separation because of our interest in the proteins responsible for plastification of cementitious systems such as mortar. The solubilized proteins were separated chromatographically via ion exchange chromatography (IEX) and the subsequently desalted protein fractions were tested for purity by isoelectric focusing (IEF).
酪蛋白用作粘合剂或涂料中的添加剂，因此具有独特的性质，这可能基于复杂的全酪蛋白混合物中某些亚蛋白的性质。因此，为了获得足够量的蛋白质亚组分α-、β-和κ-酪蛋白以利用快速蛋白质液相色谱（FPLC）进行进一步测试，并在κ-酪蛋白的情况下进行预富集，以克级规模从牛奶中分离全酪蛋白（CN）。由于我们对负责胶凝体系（如砂浆）增塑的蛋白质感兴趣，因此使用质量不同于乳制品级酪蛋白的建筑化学级酪蛋白进行分离。通过离子交换色谱（IEX）对溶解的蛋白质进行色谱分离，随后通过等电聚焦（IEF）测试脱盐后的蛋白质组分的纯度。
