Gowda Nagaraj M, Goswami Usha, Khan M Islam
Gene lab, National Institute of Oceanography, Goa 403 004, India; Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, India.
J Invertebr Pathol. 2008 Oct;99(2):141-5. doi: 10.1016/j.jip.2008.04.003. Epub 2008 Apr 18.
In invertebrates, cellular and humoral components are evolved to maintain their body immunity and integrity. Both these factors respond to different antigens such as microorganisms, vertebrate erythrocytes and foreign proteins. In this article, we report a study of a lectin (HSL) involved in immune response in the echinoderm, sea cucumber (Holothuria scabra). Correlative studies indicate that the expression of this defensive lectin is induced by bacterial challenge, wherein cell wall glycoconjugates of bacteria are involved in lectin induction. HSL showed strong broad spectrum antibacterial activity against both gram-positive and gram-negative bacteria. Under in vitro conditions, purified HSL mediate agglutination of the test bacteria, there by indicating a possible mode of action in physiological situation.
在无脊椎动物中，细胞和体液成分不断进化以维持身体的免疫力和完整性。这两种因素对不同的抗原做出反应，如微生物、脊椎动物红细胞和外来蛋白质。在本文中，我们报告了一项关于参与棘皮动物海参（糙海参）免疫反应的凝集素（HSL）的研究。相关研究表明，这种防御性凝集素的表达是由细菌攻击诱导的，其中细菌的细胞壁糖缀合物参与了凝集素的诱导。HSL对革兰氏阳性菌和革兰氏阴性菌均表现出强大的广谱抗菌活性。在体外条件下，纯化的HSL介导受试细菌的凝集，从而表明其在生理情况下可能的作用方式。
