Van Gerven Nani, De Greve Henri, Hernalsteens Jean-Pierre
Onderzoeksgroep Genetische Virologie, Vrije Universiteit Brussel, B-1050 Brussels, Belgium.
Vet Microbiol. 2008 Oct 15;131(3-4):369-75. doi: 10.1016/j.vetmic.2008.04.001. Epub 2008 May 23.
We examined the potential of inactivated Salmonella strains to induce protective antibodies against two adhesins of pathogenic Escherichia coli. The receptor-binding domains of the F17a-G adhesin of F17a fimbriae and of the FimH adhesin of type 1 fimbriae were fused to the translocator domain of the autotransporter AIDA-I. An IgG response against F17a-G or FimH was induced after immunization of mice with acetone-inactivated Salmonella displaying the corresponding fimbrial receptor-binding domain. These sera inhibit in vitro agglutination of erythrocytes by E. coli carrying these fimbriae. Our results demonstrate that induced and subsequently acetone-inactivated Salmonella are useful delivery vehicles for the stimulation of an IgG antibody response against heterologous antigens.
我们研究了灭活沙门氏菌菌株诱导针对致病性大肠杆菌两种粘附素的保护性抗体的潜力。将F17a菌毛的F17a - G粘附素的受体结合结构域和1型菌毛的FimH粘附素的受体结合结构域与自转运蛋白AIDA - I的转运结构域融合。用展示相应菌毛受体结合结构域的丙酮灭活沙门氏菌免疫小鼠后，可诱导产生针对F17a - G或FimH的IgG反应。这些血清可在体外抑制携带这些菌毛的大肠杆菌对红细胞的凝集作用。我们的结果表明，诱导后再经丙酮灭活的沙门氏菌是刺激针对异源抗原产生IgG抗体反应的有效递送载体。
