一种从骨髓基质细胞中获取神经球的简单高效方法。
A simple and efficient method for deriving neurospheres from bone marrow stromal cells.
作者信息
Yang Qin, Mu Jun, Li Qi, Li Ao, Zeng Zhilei, Yang Jun, Zhang Xiaodong, Tang Jin, Xie Peng
机构信息
Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, The Key Laboratory of Diagnostic Medicine Designated by the Ministry of Education, 1 Yixueyuan Road, Chongqing 400016, China.
出版信息
Biochem Biophys Res Commun. 2008 Aug 8;372(4):520-4. doi: 10.1016/j.bbrc.2008.05.039. Epub 2008 May 23.
Bone marrow stromal cells (MSCs) can be differentiated into neuronal and glial-like cell types under appropriate experimental conditions. However, previously reported methods are complicated and involve the use of toxic reagents. Here, we present a simplified and nontoxic method for efficient conversion of rat MSCs into neurospheres that express the neuroectodermal marker nestin. These neurospheres can proliferate and differentiate into neuron, astrocyte, and oligodendrocyte phenotypes. We thus propose that MSCs are an emerging model cell for the treatment of a variety of neurological diseases.
在适当的实验条件下，骨髓基质细胞（MSCs）可分化为神经元样和神经胶质样细胞类型。然而，先前报道的方法很复杂，且涉及使用有毒试剂。在此，我们提出一种简单且无毒的方法，可有效将大鼠MSCs转化为表达神经外胚层标志物巢蛋白的神经球。这些神经球能够增殖并分化为神经元、星形胶质细胞和少突胶质细胞表型。因此，我们认为MSCs是治疗多种神经疾病的一种新兴模型细胞。
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