Zheng Jinghui, Liang Jian, Deng Xin, Chen Xiaofeng, Wu Fasheng, Zhao Xiaofang, Luo Yuan, Fu Lei, Jiang Zuling
Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.
Neural Regen Res. 2012 Jun 25;7(18):1370-7.
Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.
我们的初步研究证实，补阳还五汤的活性成分区域，包括生物碱、多糖、苷元、糖苷和挥发油，可诱导骨髓间充质干细胞分化为神经元。丝裂原活化蛋白激酶信号通路被确定为这一分化过程的关键途径之一。本研究表明，分化后磷酸化细胞外信号调节蛋白激酶和磷酸化p38蛋白表达增加。细胞信号通路阻断剂PD98059和SB203580分别抑制丝裂原活化蛋白激酶信号通路中的细胞外信号调节蛋白激酶和p38。用补阳还五汤活性成分区域处理后，骨髓间充质干细胞中神经元标志物神经元特异性烯醇化酶和神经干细胞标志物巢蛋白的mRNA和蛋白表达降低。实验结果表明，丝裂原活化蛋白激酶信号通路中的细胞外信号调节蛋白激酶和p38参与了补阳还五汤活性成分区域诱导的骨髓间充质干细胞向神经元样细胞的分化。
