Bridgen A
Moredun Research Institute, Edinburgh, U.K.
Arch Virol. 1991;117(3-4):183-92. doi: 10.1007/BF01310764.
The gammaherpesvirus Alcelaphine herpesvirus 1 (AHV-1), indigenous to the wildebeest (Connochaetes species), is a causative agent of the fatal lymphoproliferative disease malignant catarrhal fever in cattle and deer. The genome of the attenuated WC11 isolate of AHV-1 has previously been shown to possess a region of unique DNA together with multiple direct repeat sequences. Approximately 70% of the genome of WC11 has now been cloned into plasmid or bacteriophage vectors and these clones have been used in hybridisation experiments to construct a restriction endonuclease map of the WC11 unique DNA with respect to BamHI. EcoRI, SalI, SmaI and XhoI. The map allows the size of the unique region of the WC11 genome to be estimated as 130 kbp and thus the entire genome as 155-160 kbp. The results confirm a terminal location for the repeat sequences.
γ疱疹病毒1型（AHV-1）原产于牛羚（角马属物种），是牛和鹿致命性淋巴细胞增生性疾病恶性卡他热的病原体。此前已证明，AHV-1减毒WC11分离株的基因组含有一个独特DNA区域以及多个直接重复序列。目前，WC11基因组约70%已被克隆到质粒或噬菌体载体中，这些克隆已用于杂交实验，以构建WC11独特DNA相对于BamHI、EcoRI、SalI、SmaI和XhoI的限制性内切酶图谱。该图谱显示，WC11基因组独特区域的大小估计为130 kbp，因此整个基因组大小为155-160 kbp。结果证实了重复序列位于末端。
