Kirchhof Kristin, Groth Thomas
Biomedical Materials Group, Department of Pharmaceutics and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle(Saale), Germany.
Clin Hemorheol Microcirc. 2008;39(1-4):247-51.
The layer-by-layer technique was used to build-up polyelectrolyte multilayers (PEMs) composed of heparin, an anionic glycosaminoglycan (GAG) and chitosan, a cationic biodegradable polysaccharide on model biomaterial surfaces. The surface coatings shall control adhesion of cells and thus their subsequent proliferation and differentiation. PEMs were characterized physicochemically by static contact angle and quartz crystal microbalance (QCM) measurements. Variations in procedure parameters such as the pH value of the solutions were crucial to the formation process and surface properties in terms of wettability and mass increase. Cell-surface interactions were studied with human fibroblast on PEMs. It was found that the pH value of solutions had a strong impact on cell adhesion making surfaces extremely cytophobic or moderately cytophilic. Adsorption of fibronectin to the terminal heparin layer could be used to increase cell adhesion in a concentration-dependent manner.
采用层层组装技术在模型生物材料表面构建由肝素（一种阴离子糖胺聚糖（GAG））和壳聚糖（一种阳离子可生物降解多糖）组成的聚电解质多层膜（PEM）。表面涂层应控制细胞的黏附，从而控制其随后的增殖和分化。通过静态接触角和石英晶体微天平（QCM）测量对PEM进行物理化学表征。诸如溶液pH值等程序参数的变化对于形成过程以及润湿性和质量增加方面的表面性质至关重要。用人成纤维细胞研究了细胞与表面的相互作用。发现溶液的pH值对细胞黏附具有强烈影响，使表面具有极强的细胞疏水性或适度的细胞亲水性。纤连蛋白吸附到末端肝素层可用于以浓度依赖的方式增加细胞黏附。
