Tabata Setsuko, Iida Kenji, Kimura Keisuke, Iwasaki Yumiko, Nakazato Mitsuo, Kamata Kunihiro, Hirokado Masako
Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
Shokuhin Eiseigaku Zasshi. 2008 Apr;49(2):111-5. doi: 10.3358/shokueishi.49.111.
Ochratoxin A (OTA), ochratoxin B (OTB) and citrinin (CIT) in commercial foods were simultaneously determined and confirmed with high-performance liquid chromatography (HPLC) and liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). The samples examined were made up of cereal, fruit, coffee, and cacao products. The limits of quantification (S/N> or =10) of OTA, OTB and CIT were 0.1 microg/kg or less. Aflatoxins (AF), deoxynivalenol (DON) and fumonisins were also surveyed. Of 157 samples examined, 44 were contaminated with OTA at levels of 0.11 to 4.0 microg/kg. At least 2 positive samples were labeled as domestics. In most positive samples, the OTA level was low, less than 1 microg/kg. The highest incidence of OTA was observed in cacao powder (10/12), followed by instant coffee (5/7), cocoa (5/8) and raisin (6/13). OTB was found in fruit and cacao products containing relatively high levels of OTA. Co-occurrence of OTA, CIT and DON was found in cereal products, and co-occurrence of OTA and AF was found in cacao products. Approximately 30% of naturally contaminated OTA in roasted coffee bean moved into the extract solution when brewed with paper filter.
采用高效液相色谱法（HPLC）以及液相色谱-串联质谱法（LC/MS/MS）对市售食品中的赭曲霉毒素A（OTA）、赭曲霉毒素B（OTB）和桔青霉素（CIT）进行了同时测定和确证。所检测的样品包括谷物、水果、咖啡和可可制品。OTA、OTB和CIT的定量限（S/N≥10）为0.1微克/千克或更低。同时还对黄曲霉毒素（AF）、脱氧雪腐镰刀菌烯醇（DON）和伏马菌素进行了检测。在所检测的157份样品中，有44份被OTA污染，污染水平为0.11至4.0微克/千克。至少有2份阳性样品标注为国产。在大多数阳性样品中，OTA水平较低，低于1微克/千克。OTA检出率最高的是可可粉（10/12），其次是速溶咖啡（5/7）、可可（5/8）和葡萄干（6/13）。在含有相对较高水平OTA的水果和可可制品中发现了OTB。在谷物制品中发现了OTA、CIT和DON的共存情况，在可可制品中发现了OTA和AF的共存情况。当用纸质滤器冲泡时, 烘焙咖啡豆中约30%天然污染的OTA会转移到萃取液中。
