Vladimirov Stoyan B, Stamatova Iva V, Atanasova Pepa K, Baltadjiev Georgi, Borisov Ivan
Department of Operative Dentistry and Endodontics, Faculty of Dental Medicine, Plovdiv, Bulgaria.
Folia Med (Plovdiv). 2007;49(3-4):70-4.
The aim of the present experimental study was to follow up the connective tissue response after using ProRoot MTA and Titan cement to repair furcation perforations in dogs.
Four animals aged 12 to 18 months were used in the study. Perforation defects were created in the center of the pulp chamber of mandibular premolars P2, P3 and P4, right and left. The defects on the left side were repaired with ProRoot MTA, and those on the right side--with Titan cement in all dogs. After 30 days bone fragments with teeth included were fixed in formalin and decalcified in 50% formic acid. Serial sections of 10 microm thickness were prepared, stained with hematoxylin and eosin and studied under light microscopy.
The connective tissue response in the Titan cement repaired teeth was a fibrous capsule in contact with the material, with single or aggregated lymphocytes seen in the vicinity. The response to ProRoot MTA was similar, but the fibrous capsule was thinner and without any aggregation of lymphocytes.
The connective tissue response was similar for both tested materials. The tissues tolerated both of them well; it formed a fibrous capsule, which is indicative of the start of a healing process.
本实验研究的目的是追踪使用ProRoot MTA和Titan水门汀修复犬类根分叉穿孔后结缔组织的反应。
本研究使用了4只年龄在12至18个月的动物。在下颌前磨牙P2、P3和P4牙髓腔中央，左右两侧均制造穿孔缺损。在所有犬类中，左侧的缺损用ProRoot MTA修复，右侧的缺损用Titan水门汀修复。30天后，将包含牙齿的骨碎片固定在福尔马林中，并用50%甲酸脱钙。制备10微米厚的连续切片，用苏木精和伊红染色，并在光学显微镜下观察。
Titan水门汀修复牙齿的结缔组织反应是与材料接触处形成纤维性囊，在其附近可见单个或聚集的淋巴细胞。对ProRoot MTA的反应相似，但纤维性囊较薄且无淋巴细胞聚集。
两种受试材料的结缔组织反应相似。组织对两者耐受性良好；形成了纤维性囊，这表明愈合过程开始。
