神经生长因子诱导表达trk原癌基因产物的非洲爪蟾卵母细胞减数分裂成熟
Induction by NGF of meiotic maturation of Xenopus oocytes expressing the trk proto-oncogene product.
作者信息
Nebreda A R, Martin-Zanca D, Kaplan D R, Parada L F, Santos E
机构信息
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
出版信息
Science. 1991 Apr 26;252(5005):558-61. doi: 10.1126/science.1850550.
The effect of nerve growth factor (NGF) was assessed in Xenopus oocytes expressing the human trk proto-oncogene product, p140prototrk. Oocytes injected with trk messenger RNA expressed polypeptides recognized by antibodies to the trk gene product. Exposure of these oocytes to nanomolar amounts of NGF resulted in specific surface binding of 125I-labeled NGF, tyrosine phosphorylation of p140prototrk, and meiotic maturation, as determined by germinal vesicle breakdown and maturation promoting factor (p34cdc2) kinase activation. Thus the trk proto-oncogene product can act as a receptor for NGF in a functionally productive manner.
在表达人类trk原癌基因产物p140prototrk的非洲爪蟾卵母细胞中评估了神经生长因子（NGF）的作用。注射了trk信使核糖核酸的卵母细胞表达了能被trk基因产物抗体识别的多肽。将这些卵母细胞暴露于纳摩尔量的NGF中，会导致125I标记的NGF发生特异性表面结合、p140prototrk的酪氨酸磷酸化以及减数分裂成熟，这是通过生发泡破裂和成熟促进因子（p34cdc2）激酶激活来确定的。因此，trk原癌基因产物能够以功能有效的方式充当NGF的受体。
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