Ladhar-Chaabouni Rim, Mokdad-Gargouri Raja, Denis Françoise, Hamza-Chaffai Amel
IPEIS, Ecotoxicologie Marine, UR 09-03, Sfax, Tunisia.
Mol Biol Rep. 2009 May;36(5):1007-14. doi: 10.1007/s11033-008-9274-8. Epub 2008 May 28.
cDNA probes have been developed for subsequent use in monitoring the cadmium exposure of the clam Ruditapes decussatus and the cockle Cerastoderma glaucum using metallothionein (MT) gene expression in different tissues of these species. Two partial MT cDNAs were isolated from Ruditapes decussatus and Cerastoderma glaucum. The identification of the nucleotide sequences showed that the cDNAs consist of 480 bp coding 72 amino acid proteins containing 21 cysteine residues organized in Cys-X-Cys motifs as classically described for MTs. The induction of MT gene expression in CdCl(2) treated bivalves was confirmed by dot blot analysis and suggests a potential specific tissue expression rate.
已经开发出了互补DNA（cDNA）探针，随后可用于利用这些物种不同组织中的金属硫蛋白（MT）基因表达来监测蛤仔（Ruditapes decussatus）和鸟蛤（Cerastoderma glaucum）的镉暴露情况。从蛤仔和鸟蛤中分离出了两个MT cDNA片段。核苷酸序列鉴定表明，这些cDNA由480个碱基对组成，编码含72个氨基酸的蛋白质，其中有21个半胱氨酸残基，以Cys-X-Cys基序排列，这是MT的经典描述方式。通过斑点印迹分析证实了氯化镉处理的双壳贝类中MT基因表达的诱导，并表明存在潜在的特定组织表达率。
