Sarabi Arezou S, Shen Hui, Wang Yun, Hoffer Barry J, Bäckman Cristina M
Cellular Neurobiology Branch, National Institute on Drug Abuse, National Institutes of Health, Baltimore, Maryland 21224, USA.
J Neurosci Res. 2008 Oct;86(13):2912-24. doi: 10.1002/jnr.21734.
Ischemic stress in the brain causes acute and massive cell death in the targeted core area followed by a second phase of damage in the neighboring penumbra. The purpose of this study was to examine the global gene expression patterns in the penumbra, because the ischemic lesion in this region could be rescued by restoration of blood flow and other protective therapies. Adult C57Bl/6 mice were subjected to a 90-min middle cerebral artery occlusion (MCAO). Laser capture microdissection (LCM) was used for tissue dissection at 4 and 24 hr after reperfusion. Sham-operated animals were used as controls. Gene expression in the penumbra was examined by using microarray analysis and quantitative RT-PCR. In agreement with previous reports, most genes were down-regulated at 4 hr after the onset of reperfusion in the ischemic penumbra compared with controls. In contrast, at 24 hr after reperfusion, most genes were up-regulated in the ischemic penumbra. Several genes not previously reported to be associated with ischemia were found. The gene lists generated in this study will help us to understand better the spatial and temporal distribution of molecules involved in the ischemic cascade. Published 2008 Wiley-Liss, Inc.
大脑中的缺血应激会导致目标核心区域出现急性大量细胞死亡，随后在邻近的半暗带发生第二阶段损伤。本研究的目的是检测半暗带中的整体基因表达模式，因为该区域的缺血性损伤可通过恢复血流及其他保护性治疗得以挽救。成年C57Bl/6小鼠接受90分钟的大脑中动脉闭塞（MCAO）。在再灌注后4小时和24小时，使用激光捕获显微切割（LCM）进行组织切割。假手术动物用作对照。通过微阵列分析和定量RT-PCR检测半暗带中的基因表达。与先前报道一致，与对照相比，缺血半暗带再灌注开始后4小时大多数基因表达下调。相反，再灌注后24小时，缺血半暗带中的大多数基因表达上调。发现了一些先前未报道与缺血相关的基因。本研究生成的基因列表将有助于我们更好地理解缺血级联反应中涉及分子的时空分布。2008年由Wiley-Liss公司出版。
