使用三种不同的手性选择剂作为浸渍试剂，通过直接薄层色谱法将阿替洛尔和普萘洛尔拆分为对映体。
Direct TLC resolution of atenolol and propranolol into their enantiomers using three different chiral selectors as impregnating reagents.
作者信息
Bhushan Ravi, Tanwar Shivani
机构信息
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
出版信息
Biomed Chromatogr. 2008 Sep;22(9):1028-34. doi: 10.1002/bmc.1025.
DOI:10.1002/bmc.1025
PMID:18506901
Abstract
Direct resolution of racemic atenolol and propranolol into their enantiomers was achieved by normal phase TLC on silica gel plates impregnated with optically pure L-tartaric acid, (R)-mandelic acid and (-)-erythromycin as chiral selectors. Different solvent systems were worked out to resolve the enantiomers. Spots were detected using iodine vapour. The TLC method was validated for linearity, limit of detection and limit of quantification. The influence of pH, temperature and concentration of chiral selector was studied.
摘要
通过在浸渍有光学纯L-酒石酸、(R)-扁桃酸和(-)-红霉素作为手性选择剂的硅胶板上进行正相薄层色谱法，实现了外消旋阿替洛尔和普萘洛尔直接拆分为其对映体。设计了不同的溶剂系统来拆分对映体。使用碘蒸气检测斑点。对该薄层色谱法进行了线性、检测限和定量限的验证。研究了pH、温度和手性选择剂浓度的影响。
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