Wang Lifeng, Liu Baorui, Wang Tingting, Ding Yitao, Qian Xiaoping, Zhao Yang
Department of Oncology, Drum Tower Hospital Affiliated to Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China.
Anticancer Res. 2008 Mar-Apr;28(2A):1085-91.
The aim of this study was to characterize cell-free mRNA in malignant effusions and evaluate its correlation with the sensitivity of primary tumor cells to cisplatin and 5-fluorouracil (5-FU).
Malignant effusions were collected from 43 patients and ERCC1 and TS mRNA transcripts in both the supernatants and tumor cells were characterized by real-time quantitative PCR. The sensitivity of primary tumor cells to cisplatin and 5-FU was analyzed using an ATP-TCA assay.
Cell-free ERCC1 and TS mRNA transcripts were observed in 42 out of 43 specimens, with variable levels comparing gene expression patterns in paired-tumor cells (p < 0.0001, p = 0.02, respectively). TS mRNA levels in the supernatants were inversely correlated with the in vitro sensitivity of cancer cells to 5-FU in the gastrointestinal group (p = 0.002).
The cell-free mRNA transcripts in malignant effusions were highly detectable and cell-free TS mRNA in gastrointestinal cancer patients were strongly associated with the sensitivity of primary cancer cells to 5-FU in vitro.
本研究旨在对恶性胸腔积液中的游离mRNA进行特征分析，并评估其与原发性肿瘤细胞对顺铂和5-氟尿嘧啶（5-FU）敏感性的相关性。
收集43例患者的恶性胸腔积液，通过实时定量PCR对上清液和肿瘤细胞中的ERCC1和TS mRNA转录本进行特征分析。采用ATP-TCA法分析原发性肿瘤细胞对顺铂和5-FU的敏感性。
43份标本中有42份检测到游离的ERCC1和TS mRNA转录本，比较配对肿瘤细胞中的基因表达模式时发现其水平存在差异（分别为p < 0.0001，p = 0.02）。胃肠道组中，上清液中TS mRNA水平与癌细胞对5-FU的体外敏感性呈负相关（p = 0.002）。
恶性胸腔积液中的游离mRNA转录本易于检测，胃肠道癌患者的游离TS mRNA与原发性癌细胞对5-FU的体外敏感性密切相关。
