Monleón Daniel, Morales José Manuel, Gonzalez-Darder José, Talamantes Fernando, Cortés Oscar, Gil-Benso Rosario, López-Ginés Concepción, Cerdá-Nicolás Miguel, Celda Bernardo
Fundacion de Investigacion del Hospital Clinico Universitario de Valencia, Valencia, Spain.
J Proteome Res. 2008 Jul;7(7):2882-8. doi: 10.1021/pr800110a. Epub 2008 May 29.
Meningiomas are neoplasms that arise from the leptomeningeal covering of the brain and spinal cord, accounting for 15%-20% of CNS tumors. The WHO classifies meningiomas into three histological grades: benign, atypical, and anaplasic in accordance with the clinical prognosis. Atypical and anaplasic meningiomas tend to recur. Sometimes, meningiomas with histological diagnosis of benign meningioma show clinical characteristics of atypical meningioma. In this context, high-resolution magic-angle spinning (HR-MAS) spectroscopy of intact tissue from brain tumor biopsies has shown great potential as a support diagnostic tool. In this work, we show differences between benign and atypical meningiomas in HR-MAS molecular profiles of meningioma biopsies. Metabolic differences between meningioma grades include changes in the levels of glutathione. Glutathione role in cancer is still unclear, as it may act both as protective and pathogenic factor. Glutamine and glutamate, which are related to glutathione metabolism and have been associated with tumor recurrence, are also increased in atypical meningiomas. Other metabolites associated with tumor malignancy that show statistically significant differences between benign and atypical meningiomas include phosphocholine and phosphoethanolamine. Overall, this work suggests that the additional information obtained by NMR metabolomics applied to biopsies of human meningiomas may be useful for assessing tumor grade and determining optimum treatment strategies.
脑膜瘤是起源于脑和脊髓软脑膜被覆层的肿瘤，占中枢神经系统肿瘤的15%-20%。世界卫生组织根据临床预后将脑膜瘤分为三个组织学级别：良性、非典型性和间变性。非典型性和间变性脑膜瘤容易复发。有时，组织学诊断为良性脑膜瘤的脑膜瘤表现出非典型性脑膜瘤的临床特征。在这种情况下，脑肿瘤活检完整组织的高分辨率魔角旋转（HR-MAS）光谱作为一种辅助诊断工具显示出巨大潜力。在这项工作中，我们展示了脑膜瘤活检的HR-MAS分子谱中良性和非典型性脑膜瘤之间的差异。脑膜瘤不同级别之间的代谢差异包括谷胱甘肽水平的变化。谷胱甘肽在癌症中的作用仍不清楚，因为它可能既作为保护因素又作为致病因素起作用。与谷胱甘肽代谢相关且与肿瘤复发有关的谷氨酰胺和谷氨酸在非典型性脑膜瘤中也增加。在良性和非典型性脑膜瘤之间显示出统计学显著差异的其他与肿瘤恶性程度相关的代谢物包括磷酸胆碱和磷酸乙醇胺。总体而言，这项工作表明，通过应用于人类脑膜瘤活检的核磁共振代谢组学获得的额外信息可能有助于评估肿瘤级别并确定最佳治疗策略。
