Lai Yu-Hung, Wang Hwei-Zu, Lin Chang-Ping, Hong Show-Jen, Chang Shun-Jen
Department of Ophthalmology, Kaohsiung Medical University Hospital and Kaohsiung Medical University, Kaohsiung, Taiwan.
Kaohsiung J Med Sci. 2008 May;24(5):254-61. doi: 10.1016/S1607-551X(08)70150-5.
The objective was to study the effects of endothelin-1 (ET1) on corneal wound healing after photorefractive keratectomy (PRK) in rabbit corneas. Following PRK, 18 New Zealand white rabbits were treated with ET1 in the right eyes and with phosphate-buffered salt solution (PBS) in the left eyes. Corneal epithelial wound size, corneal haze and corneal thickness were recorded. Corneal extracellular matrixes, including collagen types 3, 4 and 7, chondroitin sulfate and fibronectin, were investigated using immunohistochemistry study. ET1 increased the rate of healing of corneal epithelial wounds in rabbits. Anti-fibronectin fluorescence was present at week 12 and week 24 in ET1-treated eyes but not in the control eyes. There were no significant differences in corneal haze, corneal thickness and changes in other extracellular matrixes between ET1- and PBS-treated eyes. ET1 can enhance the deposition of fibronectin in corneal stroma and promote corneal epithelial wound healing after PRK. The increase in fibronectin probably explains the increased healing rate of corneal epithelial wounds.
目的是研究内皮素-1（ET1）对兔眼角膜屈光性角膜切削术（PRK）后角膜伤口愈合的影响。PRK术后，18只新西兰白兔右眼用ET1治疗，左眼用磷酸盐缓冲盐溶液（PBS）治疗。记录角膜上皮伤口大小、角膜混浊和角膜厚度。采用免疫组织化学研究方法，对角膜细胞外基质，包括3型、4型和7型胶原、硫酸软骨素和纤连蛋白进行研究。ET1提高了兔角膜上皮伤口的愈合速度。在ET1治疗组眼中，第12周和第24周出现抗纤连蛋白荧光，而对照组眼中未出现。ET1治疗组和PBS治疗组眼在角膜混浊、角膜厚度及其他细胞外基质变化方面无显著差异。ET1可增强角膜基质中纤连蛋白的沉积，并促进PRK术后角膜上皮伤口愈合。纤连蛋白的增加可能解释了角膜上皮伤口愈合速度的提高。
