Chien Jui-Hung, Yang Ching-Hao, Chen Ping-Hei, Yang Chii-Rong, Lin Chin-Shen, Wang Huei
Department of Mechanical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, Taiwan, 106.
Front Biosci. 2008 May 1;13:4756-64. doi: 10.2741/3037.
This study presents a novel method for DNA detection with multi-layer AuNPs to enhance overall detection sensitivity. This essay achieves not only an innovative radio-frequency biosensor but also a critical signal amplification methodology. Results show that bandwidth change for multi-layer AuNP with hybridization of DNA exceeds that for the double-layer AuNP up to 0.5 GHz. Furthermore, the developed biosensor detection limit for the DNA set employed in this essay is currently 10 pM. A single base-pair mutation of the wild-type target DNA could be distinguished from the perfect match target DNA at the melting temperature of 47 degrees C with a temperature controlling system. Experimental results in this study indicate that the proposed biosensor and the developed amplification methodology are successful. As health care becomes much more essential in modern life, this biosensor has potential applications in a screening kit for recognizing, sensing, and quantifying biomolecules in real samples.
本研究提出了一种使用多层金纳米颗粒进行DNA检测的新方法，以提高整体检测灵敏度。本文不仅实现了一种创新的射频生物传感器，还实现了一种关键的信号放大方法。结果表明，DNA杂交的多层金纳米颗粒的带宽变化比双层金纳米颗粒的带宽变化高出0.5 GHz。此外，本文所采用的生物传感器对DNA组的检测限目前为10 pM。利用温度控制系统，在47摄氏度的解链温度下，可以区分野生型目标DNA的单个碱基对突变与完全匹配的目标DNA。本研究中的实验结果表明，所提出的生物传感器和所开发的放大方法是成功的。随着医疗保健在现代生活中变得越来越重要，这种生物传感器在用于识别、传感和定量实际样品中生物分子的筛查试剂盒中具有潜在应用。
