Tang Yew Chung, Wan Guoqiang, Ng Jin Kiat, Ajikumar Parayil Kumaran, Too Heng-Phon
Singapore-MIT Alliance, 4 Engineering Drive 3, Singapore 117 576.
Front Biosci. 2008 May 1;13:5755-71. doi: 10.2741/3113.
Microarray technology has made it possible to simultaneously study the abundance, interactions, and functions of potentially tens of thousands of biological molecules. From its earliest use in DNA microarrays, where only nucleic acids were captured and detected on the arrays, applications of microarrays now extend to those involving biomolecules such as antibodies, proteins, peptides, and carbohydrates. In contrast to the relative robustness of DNA microarrays, the use of such chemically diverse biomolecules on microarray formats presents many challenges in their fabrication as well as application. Among the many methods that have been proposed to overcome these challenges, DNA-directed assembly (DDA) has emerged as a promising strategy for the high sensitivity and multiplexed capture and detection of various analytes. In this review, we explore the challenges faced during the design, fabrication, and utilization of protein microarrays and highlight how DDA strategies, together with other recent advances in the field, are accelerating the development of platforms available for protein microarray applications.
微阵列技术使同时研究潜在的数以万计生物分子的丰度、相互作用及功能成为可能。从其最早用于DNA微阵列（当时阵列上仅捕获和检测核酸）开始，微阵列的应用如今已扩展到涉及抗体、蛋白质、肽和碳水化合物等生物分子的应用。与DNA微阵列相对稳健的特性不同，在微阵列形式上使用如此化学性质多样的生物分子在其制造和应用方面带来了许多挑战。在为克服这些挑战而提出的众多方法中，DNA定向组装（DDA）已成为一种有前景的策略，用于高灵敏度和多重捕获及检测各种分析物。在本综述中，我们探讨了蛋白质微阵列设计、制造和使用过程中面临的挑战，并强调了DDA策略以及该领域的其他最新进展如何加速可用于蛋白质微阵列应用的平台的开发。
