Reutzel-Selke Anja, Pratschke Johann, Martins Paulo N, Denecke Christian, Jurisch Anke, Kotsch Katja, Pascher Andreas, Neuhaus Peter, Tullius Stefan G
Department of General, Visceral, and Transplant Surgery, Charité, Campus Virchow-Clinic, Universitätsmedizin Berlin, Berlin, Germany.
Kidney Int. 2008 Sep;74(5):622-30. doi: 10.1038/ki.2008.208. Epub 2008 May 28.
Ischemic preconditioning directly protects organs from subsequent non-specific injuries. To test for systemic protective effects kidneys from F-344 donor rats went through a short warm ischemic time. Both, clamped and contralateral unclamped kidneys were procured after either a short (15 min) or long (24 h) reperfusion period and transplanted into Lewis rats following a prolonged cold ischemia. To test for transferable effects serum from preconditioned rats was infused either into native donors or recipients. Following a short reperfusion interval protective effects were only evident in previously clamped grafts. However, after a long reperfusion interval protective effects were observed in previously clamped and contralateral unclamped kidneys promoting improved survival, structure, function and reduced inflammation. These effects were not related to heme oxygenase-1 induction or neural transmission as heme oxygenase-1 inhibition or denervation prior to preconditioning did not affect organ protection. These results show that renal ischemic preconditioning is associated with time-dependent local and systemically transferable protection.
缺血预处理可直接保护器官免受随后的非特异性损伤。为了测试全身保护作用，对F-344供体大鼠的肾脏进行了短时间的热缺血处理。在短（15分钟）或长（24小时）再灌注期后，获取夹闭的肾脏和对侧未夹闭的肾脏，并在长时间冷缺血后移植到Lewis大鼠体内。为了测试可转移的效应，将预处理大鼠的血清注入天然供体或受体。在短时间再灌注间隔后，保护作用仅在先前夹闭的移植物中明显。然而，在长时间再灌注间隔后，在先前夹闭的肾脏和对侧未夹闭的肾脏中观察到保护作用，促进了存活率提高、结构改善、功能改善和炎症减轻。这些效应与血红素加氧酶-1诱导或神经传递无关，因为在预处理前抑制血红素加氧酶-1或去神经支配并不影响器官保护。这些结果表明，肾脏缺血预处理与时间依赖性的局部和全身可转移保护有关。
