• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大鼠肾脏中的金属蛋白酶meprin β：肾小球定位及在肾小球肾炎中的差异表达

Metalloprotease meprin beta in rat kidney: glomerular localization and differential expression in glomerulonephritis.

作者信息

Oneda Beatrice, Lods Nadège, Lottaz Daniel, Becker-Pauly Christoph, Stöcker Walter, Pippin Jeffrey, Huguenin Maya, Ambort Daniel, Marti Hans-Peter, Sterchi Erwin E

机构信息

Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland.

出版信息

PLoS One. 2008 May 28;3(5):e2278. doi: 10.1371/journal.pone.0002278.

DOI:10.1371/journal.pone.0002278
PMID:18509531
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2386549/
Abstract

Meprin (EC 3.4.24.18) is an oligomeric metalloendopeptidase found in microvillar membranes of kidney proximal tubular epithelial cells. Here, we present the first report on the expression of meprin beta in rat glomerular epithelial cells and suggest a potential involvement in experimental glomerular disease. We detected meprin beta in glomeruli of immunostained rat kidney sections on the protein level and by quantitative RT-PCR of laser-capture microdissected glomeruli on the mRNA level. Using immuno-gold staining we identified the membrane of podocyte foot processes as the main site of meprin beta expression. The glomerular meprin beta expression pattern was altered in anti-Thy 1.1 and passive Heymann nephritis (PHN). In addition, the meprin beta staining pattern in the latter was reminiscent of immunostaining with the sheep anti-Fx1A antiserum, commonly used in PHN induction. Using Western blot and immunoprecipitation assays we demonstrated that meprin beta is recognized by Fx1A antiserum and may therefore represent an auto-antigen in PHN. In anti-Thy 1.1 glomerulonephritis we observed a striking redistribution of meprin beta in tubular epithelial cells from the apical to the basolateral side and the cytosol. This might point to an involvement of meprin beta in this form of glomerulonephritis.

摘要

膜金属蛋白酶（EC 3.4.24.18）是一种寡聚金属内肽酶，存在于肾近端小管上皮细胞的微绒毛膜中。在此，我们首次报道了膜金属蛋白酶β在大鼠肾小球上皮细胞中的表达，并提示其可能参与实验性肾小球疾病。我们通过免疫染色在大鼠肾切片的肾小球中检测到了膜金属蛋白酶β的蛋白水平，并通过对激光捕获显微切割的肾小球进行定量逆转录聚合酶链反应检测到了其mRNA水平。使用免疫金染色，我们确定足细胞足突膜是膜金属蛋白酶β表达的主要部位。在抗Thy 1.1和被动型Heymann肾炎（PHN）中，肾小球膜金属蛋白酶β的表达模式发生了改变。此外，后者的膜金属蛋白酶β染色模式让人联想到常用于诱导PHN的羊抗Fx1A抗血清的免疫染色。使用蛋白质印迹和免疫沉淀分析，我们证明膜金属蛋白酶β可被Fx1A抗血清识别，因此可能是PHN中的一种自身抗原。在抗Thy 1.1肾小球肾炎中，我们观察到膜金属蛋白酶β在肾小管上皮细胞中从顶端向基底外侧和细胞质发生了显著的重新分布。这可能表明膜金属蛋白酶β参与了这种形式的肾小球肾炎。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/5a78034cdc71/pone.0002278.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/e8589283ea85/pone.0002278.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/110537082858/pone.0002278.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/803dc2736039/pone.0002278.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/2db36b937364/pone.0002278.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/5025d75a5756/pone.0002278.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/5a78034cdc71/pone.0002278.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/e8589283ea85/pone.0002278.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/110537082858/pone.0002278.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/803dc2736039/pone.0002278.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/2db36b937364/pone.0002278.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/5025d75a5756/pone.0002278.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6cd/2386549/5a78034cdc71/pone.0002278.g006.jpg

相似文献

1
Metalloprotease meprin beta in rat kidney: glomerular localization and differential expression in glomerulonephritis.大鼠肾脏中的金属蛋白酶meprin β：肾小球定位及在肾小球肾炎中的差异表达
PLoS One. 2008 May 28;3(5):e2278. doi: 10.1371/journal.pone.0002278.
2
Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies.聚集蛋白、肌营养不良聚糖和抗肌萎缩蛋白在正常肾组织及实验性肾小球病中的表达
Am J Pathol. 2000 May;156(5):1749-65. doi: 10.1016/S0002-9440(10)65046-8.
3
Glomerular expression of neuronal activity-regulated pentraxin precedes the development of anti-Thy-1-induced progressive glomerulosclerosis.神经元活性调节五聚体蛋白的肾小球表达先于抗Thy-1诱导的进行性肾小球硬化的发展。
Kidney Int. 2006 Oct;70(7):1279-86. doi: 10.1038/sj.ki.5001757. Epub 2006 Aug 9.
4
Activation of nuclear factor-kappa B by podocytes in the autologous phase of passive Heymann nephritis.足细胞在被动型Heymann肾炎自身期激活核因子-κB
Kidney Int. 2001 Mar;59(3):923-31. doi: 10.1046/j.1523-1755.2001.059003923.x.
5
Antibody-induced redistribution of Heymann antigen on the surface of cultured glomerular visceral epithelial cells: possible role in the pathogenesis of Heymann glomerulonephritis.抗体诱导海曼抗原在培养的肾小球脏层上皮细胞表面重新分布：在海曼肾小球肾炎发病机制中的可能作用。
J Immunol. 1985 Oct;135(4):2409-16.
6
Immunocytochemical localization of the Heymann nephritis antigen (GP330) in glomerular epithelial cells of normal Lewis rats.海曼肾炎抗原（GP330）在正常Lewis大鼠肾小球上皮细胞中的免疫细胞化学定位。
J Exp Med. 1983 Feb 1;157(2):667-86. doi: 10.1084/jem.157.2.667.
7
Presence of immunoglobulin M antibodies around the glomerular capillaries and in the mesangium of normal and passive Heymann nephritis rats.正常及被动型海曼肾炎大鼠肾小球毛细血管周围及系膜中免疫球蛋白M抗体的存在情况。
Int J Exp Pathol. 2004 Oct;85(4):201-12. doi: 10.1111/j.0959-9673.2004.00395.x.
8
The contribution of antibody-mediated cytotoxicity and immune-complex formation to tubulointerstitial disease in passive Heymann nephritis.抗体介导的细胞毒性和免疫复合物形成在被动型海曼肾炎肾小管间质性疾病中的作用。
Clin Immunol Immunopathol. 1992 Jan;62(1 Pt 1):42-55. doi: 10.1016/0090-1229(92)90021-f.
9
Expression of type I collagen mRNA in glomeruli of rats with passive Heymann nephritis.被动型Heymann肾炎大鼠肾小球中I型胶原mRNA的表达
Kidney Int. 1993 Jan;43(1):121-7. doi: 10.1038/ki.1993.20.
10
Experimental membranous glomerulonephritis in rats. Quantitative studies of glomerular immune deposit formation in isolated glomeruli and whole animals.大鼠实验性膜性肾小球肾炎。对分离肾小球和完整动物中肾小球免疫沉积物形成的定量研究。
J Clin Invest. 1980 Jul;66(1):71-81. doi: 10.1172/JCI109837.

引用本文的文献

1
The Life of a Kidney Podocyte.肾足细胞的生命历程。
Acta Physiol (Oxf). 2025 Aug;241(8):e70081. doi: 10.1111/apha.70081.
2
Heteroaromatic Inhibitors of the Astacin Proteinases Meprin α, Meprin β and Ovastacin Discovered by a Scaffold-Hopping Approach.通过支架跳跃法发现的基质金属蛋白酶 Meprin α、Meprin β 和 Ovastacin 的杂芳族抑制剂。
ChemMedChem. 2021 Mar 18;16(6):976-988. doi: 10.1002/cmdc.202000822. Epub 2020 Dec 23.
3
LC-MS-based metabolomics analysis to identify meprin-β-associated changes in kidney tissue from mice with STZ-induced type 1 diabetes and diabetic kidney injury.

本文引用的文献

1
Disruption of glomerular basement membrane charge through podocyte-specific mutation of agrin does not alter glomerular permselectivity.通过聚集蛋白的足细胞特异性突变破坏肾小球基底膜电荷不会改变肾小球滤过选择性。
Am J Pathol. 2007 Jul;171(1):139-52. doi: 10.2353/ajpath.2007.061116.
2
Role of meprin A in renal tubular epithelial cell injury.金属蛋白酶A在肾小管上皮细胞损伤中的作用。
Kidney Int. 2007 May;71(10):1009-18. doi: 10.1038/sj.ki.5002189. Epub 2007 Mar 21.
3
The alpha and beta subunits of the metalloprotease meprin are expressed in separate layers of human epidermis, revealing different functions in keratinocyte proliferation and differentiation.
基于 LC-MS 的代谢组学分析鉴定 STZ 诱导的 1 型糖尿病和糖尿病肾病损伤小鼠肾脏组织中 meprin-β 相关变化。
Am J Physiol Renal Physiol. 2019 Oct 1;317(4):F1034-F1046. doi: 10.1152/ajprenal.00166.2019. Epub 2019 Aug 14.
4
Regulation of the alternative β-secretase meprin β by ADAM-mediated shedding.ADAM 介导的脱落调节替代β-分泌酶 meprin β。
Cell Mol Life Sci. 2019 Aug;76(16):3193-3206. doi: 10.1007/s00018-019-03179-1. Epub 2019 Jun 14.
5
Meprin β metalloproteases associated with differential metabolite profiles in the plasma and urine of mice with type 1 diabetes and diabetic nephropathy.与1型糖尿病和糖尿病肾病小鼠血浆及尿液中差异代谢物谱相关的Meprin β金属蛋白酶
BMC Nephrol. 2019 Apr 25;20(1):141. doi: 10.1186/s12882-019-1313-2.
6
Role of meprin metalloproteinases in cytokine processing and inflammation.金属蛋白酶 meprin 在细胞因子加工和炎症中的作用。
Cytokine. 2019 Feb;114:18-25. doi: 10.1016/j.cyto.2018.11.032. Epub 2018 Dec 20.
7
Undiagnosed Kidney Injury in Uninsured and Underinsured Diabetic African American Men and Putative Role of Meprin Metalloproteases in Diabetic Nephropathy.未参保及参保不足的非裔美国糖尿病男性的未诊断肾损伤以及金属蛋白酶在糖尿病肾病中的假定作用
Int J Nephrol. 2018 Apr 29;2018:6753489. doi: 10.1155/2018/6753489. eCollection 2018.
8
Meprin Metalloprotease Deficiency Associated with Higher Mortality Rates and More Severe Diabetic Kidney Injury in Mice with STZ-Induced Type 1 Diabetes.Meprin 金属蛋白酶缺乏与 STZ 诱导的 1 型糖尿病小鼠更高的死亡率和更严重的糖尿病肾脏损伤相关。
J Diabetes Res. 2017;2017:9035038. doi: 10.1155/2017/9035038. Epub 2017 Jul 19.
9
The First Scube3 Mutant Mouse Line with Pleiotropic Phenotypic Alterations.首个具有多效性表型改变的Scube3突变小鼠品系。
G3 (Bethesda). 2016 Dec 7;6(12):4035-4046. doi: 10.1534/g3.116.033670.
10
Isoform-specific interactions between meprin metalloproteases and the catalytic subunit of protein kinase A: significance in acute and chronic kidney injury.金属蛋白酶 meprin 同工型与蛋白激酶 A 催化亚基的同工型特异性相互作用：在急性和慢性肾损伤中的意义。
Am J Physiol Renal Physiol. 2015 Jan 1;308(1):F56-68. doi: 10.1152/ajprenal.00167.2014. Epub 2014 Oct 29.
金属蛋白酶meprin的α亚基和β亚基在人类表皮的不同层中表达，揭示了其在角质形成细胞增殖和分化中的不同功能。
J Invest Dermatol. 2007 May;127(5):1115-25. doi: 10.1038/sj.jid.5700675. Epub 2007 Jan 4.
4
Role for macrophage metalloelastase in glomerular basement membrane damage associated with alport syndrome.巨噬细胞金属弹性蛋白酶在与Alport综合征相关的肾小球基底膜损伤中的作用。
Am J Pathol. 2006 Jul;169(1):32-46. doi: 10.2353/ajpath.2006.050896.
5
Stage-specific action of matrix metalloproteinases influences progressive hereditary kidney disease.基质金属蛋白酶的阶段特异性作用影响进行性遗传性肾病。
PLoS Med. 2006 Apr;3(4):e100. doi: 10.1371/journal.pmed.0030100. Epub 2006 Mar 7.
6
Differential regulation of metzincins in experimental chronic renal allograft rejection: potential markers and novel therapeutic targets.金属锌蛋白酶在实验性慢性同种异体肾移植排斥反应中的差异调节：潜在标志物和新的治疗靶点
Kidney Int. 2006 Jan;69(2):358-68. doi: 10.1038/sj.ki.5000049.
7
Generation of biologically active interleukin-1beta by meprin B.膜金属蛋白酶B产生具有生物活性的白细胞介素-1β
Cytokine. 2005 Sep 7;31(5):394-403. doi: 10.1016/j.cyto.2005.06.012.
8
Meprin metalloprotease expression and regulation in kidney, intestine, urinary tract infections and cancer.
FEBS Lett. 2005 Jun 13;579(15):3317-22. doi: 10.1016/j.febslet.2005.03.045. Epub 2005 Mar 31.
9
Podocytes are firmly attached to glomerular basement membrane in kidneys with heavy proteinuria.足细胞在患有严重蛋白尿的肾脏中与肾小球基底膜紧密相连。
J Am Soc Nephrol. 2004 Oct;15(10):2611-8. doi: 10.1097/01.ASN.0000139478.03463.D9.
10
Transport of meprin subunits through the secretory pathway: role of the transmembrane and cytoplasmic domains and oligomerization.美普明亚基通过分泌途径的转运：跨膜和胞质结构域以及寡聚化的作用。
J Biol Chem. 2004 Aug 13;279(33):34856-64. doi: 10.1074/jbc.M405774200. Epub 2004 Jun 8.