Liu Lei, Piao Yue-Shan, Wang Wei, Chen Li, Wei Li-Feng, Yang Hong, Lu De-Hong
Department of Pathology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China.
Zhonghua Bing Li Xue Za Zhi. 2008 Jan;37(1):16-20.
To evaluate the alteration of subunits composition in NMDA receptor and the alterations of the expression and distribution of NMDA receptors and parvalbumin (PV)-positive neurons in focal cortical dysplasia (FCD) cortices.
Twenty cases of FCD samples (including all four subtypes of FCD) obtained during epilepsy surgery and 4 controls were analysed by immunohistochemical staining for NR1, NR2A/B and PV.
Increased expression of NR1 was detected in the giant neurons and dysmorphic neurons in FCD; while pronounced expression of NR2A/B was detected in immature neurons, giant neurons and dysmorphic neurons of FCD, especially in somata and processes of the immature neurons. Compared with the controls, FCD cortices showed prominent scattered arrangement of PV positive neurons and fibers, dramatically decreased number of PV positive interneurons and PV background staining, especially in foci of FCD II subtype.
There are increased expressions of NR1 and NR2A/B subunits in FCD abnormal neurons, as well as scattered and reduced expressions of PV positive neurons and fibers in FCD cortices.
评估局灶性皮质发育不良（FCD）皮质中N-甲基-D-天冬氨酸（NMDA）受体亚基组成的变化，以及NMDA受体和小白蛋白（PV）阳性神经元的表达和分布变化。
对癫痫手术中获取的20例FCD样本（包括FCD的所有四种亚型）和4例对照进行NR1、NR2A/B和PV的免疫组织化学染色分析。
在FCD的巨大神经元和异形神经元中检测到NR1表达增加；而在FCD的未成熟神经元、巨大神经元和异形神经元中检测到NR2A/B的明显表达，尤其是在未成熟神经元的胞体和突起中。与对照组相比，FCD皮质中PV阳性神经元和纤维呈明显散在分布，PV阳性中间神经元数量和PV背景染色显著减少，尤其是在FCD II型亚型病灶中。
FCD异常神经元中NR1和NR2A/B亚基表达增加，FCD皮质中PV阳性神经元和纤维呈散在且减少的表达。
