由超氧阴离子自由基的酸性形式——氢过氧自由基引发的NAD(P)H自氧化的稳态动力学。
Steady-state kinetics of autoxidation of NAD(P)H initiated by hydroperoxyl radical, the acid form of superoxide anion radical.
作者信息
Fujimori K, Nakajima H
机构信息
Department of Chemistry, University of Tsukuba, Ibaraki, Japan.
出版信息
Biochem Biophys Res Commun. 1991 Apr 30;176(2):846-51. doi: 10.1016/s0006-291x(05)80263-3.
Rates of autoxidation of NAD(P)H initiated by hydroperoxyl radical, the acid form of superoxide anion radical which was generated by xanthine/xanthine oxidase, followed a typical autoxidation kinetic equation. Second-order rate constants for the reactions of NADPH and NADH with hydroperoxyl radical were found to be 9.82 +/- 0.13 x 10(4) M-1s-1 and 9.26 +/- 0.58 x 10(4) M-1s-1 at 25 degrees C, respectively. Rates of the reactions between NAD(P)H and superoxide to give degraded products other than NAD(P)+ were also investigated.
由黄嘌呤/黄嘌呤氧化酶产生的超氧阴离子自由基的酸性形式——氢过氧自由基引发的NAD(P)H自氧化速率遵循典型的自氧化动力学方程。在25℃时，NADPH和NADH与氢过氧自由基反应的二级速率常数分别为9.82±0.13×10⁴ M⁻¹s⁻¹和9.26±0.58×10⁴ M⁻¹s⁻¹。还研究了NAD(P)H与超氧阴离子反应生成除NAD(P)⁺之外的降解产物的反应速率。
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