Lesiak Aleksandra, Narbutt Joanna, Kobos Józef, Sysa-Jedrzejowska Anna, Woźniacka Anna
Katedra i Klinika Dermatologii i Wenerologii Uniwersytetu Medycznego w Lodzi.
Przegl Lek. 2007;64(9):563-7.
Systemic lupus erythematosus (SLE) is a chronic, autoimmune disease with complex etiology in which genetic, hormonal and environmental factors including ultraviolet radiation play an important role. Immune and inflammatory processes participate in skin lesions development and internal organ damage. Till now most of investigations estimated the role of inflammatory and immune response in skin lesions, but only scarce data evaluate these parameters in normal appearing skin in SLE patients. The aim of our study was to determine the influence of high dose UVB on the number of HLA-DR+ and CD1a+ cells in normal appearing skin of SLE patients. The study included 20 SLE patients and 14 healthy controls. The number of HLA-DR+ and CD1a+ cells were estimated by immunohistochemical method in two skin biopsies taken from non-irradiated and from irradiated sites, 24 h after exposure to 3 MED. All skin sections were stained with antibodies directed against HLA-DR antigen and CD1a molecule. The number of HLA-DR+ was statistically significantly higher in SLE patients comparing to healthy individuals, and UVB irradiation enhanced the difference. Similarly, the mean number of CD1a+ epidermal cells was significantly higher than in controls. In healthy subjects ultraviolet radiation caused migration of these cells into dermis, thus their number was lower in the epidermis. In contrast, in SLE patients under UVB CD1a+ cell count was significantly higher. The increased number of HLA-DR+ and epidermal CD1a+ cells in SLE may suggest that enhanced antigen presentation, leading to excessive cellular immune response and inflammatory reaction development, may result in induction or photoinduction of LE specific skin lesion.
系统性红斑狼疮（SLE）是一种病因复杂的慢性自身免疫性疾病，其中遗传、激素和包括紫外线辐射在内的环境因素起着重要作用。免疫和炎症过程参与皮肤病变的发展和内脏器官的损害。到目前为止，大多数研究评估了炎症和免疫反应在皮肤病变中的作用，但只有很少的数据评估SLE患者外观正常皮肤中的这些参数。我们研究的目的是确定高剂量紫外线B对SLE患者外观正常皮肤中HLA-DR+和CD1a+细胞数量的影响。该研究包括20名SLE患者和14名健康对照。在暴露于3个最小红斑量（MED）24小时后，通过免疫组织化学方法在取自未照射部位和照射部位的两块皮肤活检组织中估计HLA-DR+和CD1a+细胞的数量。所有皮肤切片均用针对HLA-DR抗原和CD1a分子的抗体染色。与健康个体相比，SLE患者中HLA-DR+的数量在统计学上显著更高，紫外线B照射增强了这种差异。同样，CD1a+表皮细胞的平均数量也显著高于对照组。在健康受试者中，紫外线辐射导致这些细胞迁移到真皮中，因此它们在表皮中的数量较低。相比之下，在紫外线B照射下的SLE患者中，CD1a+细胞计数显著更高。SLE患者中HLA-DR+和表皮CD1a+细胞数量的增加可能表明，抗原呈递增强，导致过度的细胞免疫反应和炎症反应发展，可能导致LE特异性皮肤病变的诱发或光诱发。
