Kereïche Sami, Kouril Roman, Oostergetel Gert T, Fusetti Fabrizia, Boekema Egbert J, Doust Alexander B, van der Weij-de Wit Chantal D, Dekker Jan P
Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Biochim Biophys Acta. 2008 Sep;1777(9):1122-8. doi: 10.1016/j.bbabio.2008.04.045. Epub 2008 May 9.
Photosynthetic supercomplexes from the cryptophyte Rhodomonas CS24 were isolated by a short detergent treatment of membranes from the cryptophyte Rhodomonas CS24 and studied by electron microscopy and low-temperature absorption and fluorescence spectroscopy. At least three different types of supercomplexes of photosystem I (PSI) monomers and peripheral Chl a/c(2) proteins were found. The most common complexes have Chl a/c(2) complexes at both sides of the PSI core monomer and have dimensions of about 17x24 nm. The peripheral antenna in these supercomplexes shows no obvious similarities in size and/or shape with that of the PSI-LHCI supercomplexes from the green plant Arabidopsis thaliana and the green alga Chlamydomonas reinhardtii, and may be comprised of about 6-8 monomers of Chl a/c(2) light-harvesting complexes. In addition, two different types of supercomplexes of photosystem II (PSII) dimers and peripheral Chl a/c(2) proteins were found. The detected complexes consist of a PSII core dimer and three or four monomeric Chl a/c(2) proteins on one side of the PSII core at positions that in the largest complex are similar to those of Lhcb5, a monomer of the S-trimer of LHCII, Lhcb4 and Lhcb6 in green plants.
通过对隐藻红胞藻CS24的膜进行短时间去污剂处理，分离出了来自该隐藻的光合超复合体，并通过电子显微镜以及低温吸收和荧光光谱进行了研究。发现了至少三种不同类型的光系统I（PSI）单体与外周叶绿素a/c₂蛋白的超复合体。最常见的复合体在PSI核心单体的两侧都有叶绿素a/c₂复合体，尺寸约为17×24纳米。这些超复合体中的外周天线在大小和/或形状上与绿色植物拟南芥和绿藻莱茵衣藻的PSI-LHCI超复合体没有明显相似之处，可能由约6-8个叶绿素a/c₂捕光复合体单体组成。此外，还发现了两种不同类型的光系统II（PSII）二聚体与外周叶绿素a/c₂蛋白的超复合体。检测到的复合体由一个PSII核心二聚体和位于PSII核心一侧的三个或四个单体叶绿素a/c₂蛋白组成，在最大的复合体中，这些位置与绿色植物中LHCII的S三聚体的单体Lhcb5、Lhcb4和Lhcb6的位置相似。
