Somekh Michael G, Hsu Ken, Pitter Mark C
Institute of Biophysics, Imaging and Optical Science, School of Electrical and Electronic Engineering, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
J Opt Soc Am A Opt Image Sci Vis. 2008 Jun;25(6):1319-29. doi: 10.1364/josaa.25.001319.
Structured illumination can be employed to extend the lateral resolution of wide-field fluorescence microscopy. Since a structured illumination microscopy image is reconstructed from a series of several acquired images, we develop a modified formulation of the imaging response of the microscope and a probabilistic analysis to assess the resolution performance. We use this model to compare the fluorescence imaging performance of structured illumination techniques to confocal microscopy. Specifically, we examine the trade-off between achievable lateral resolution and signal-to-noise ratio when photon shot noise is dominant. We conclude that for a given photon budget, structured illumination invariably achieves better lateral resolution than confocal microscopy.
结构照明显微术可用于扩展宽场荧光显微镜的横向分辨率。由于结构照明显微术图像是从一系列采集的图像中重建而来，我们开发了一种显微镜成像响应的改进公式以及一种概率分析方法来评估分辨率性能。我们使用该模型将结构照明技术的荧光成像性能与共聚焦显微镜进行比较。具体而言，当光子散粒噪声占主导时，我们研究了可实现的横向分辨率与信噪比之间的权衡。我们得出结论，在给定的光子预算下，结构照明显微术始终能比共聚焦显微镜实现更好的横向分辨率。
