Gieseler R K, Röber R A, Kuhn R, Weber K, Osborn M, Peters J H
Department of Immunology, Georg August University of Göttingen, Federal Republic of Germany.
Eur J Cell Biol. 1991 Feb;54(1):171-81.
Serum-free conditions have been developed to differentiate dendritic cells from a non-adherent fraction of rat bone marrow precursors by action of the multipotential and macrophage colony-stimulating factors further supplemented with linoleic acid, vitamin E, and vitamin D3. Accessory activity was demonstrated by the high potency of the dendritic cells to stimulate autologous T cell proliferation, whereas such cells were negative for Fc receptor-dependent phagocytosis, a characteristic macrophage feature. While the dendritic cells were weakly positive for alpha-naphtylbutyrate esterase, they strongly expressed RT.1 class II antigens. Apparently, these cells represent a more differentiated phenotype since they expressed the nuclear A/C lamins. By addition of serum to the cultures, the dendritic cells developed into macrophages, which were also lamin A/C-positive as well as strongly positive for alpha-naphtylbutyrate esterase. Thus, these dendritic cells belong to the myeloid lineage, and it appears as if serum factor(s) control differentiation at a mature level. Suitable conditions could also be established for large-scale cultures of dendritic cells, which would be useful for applications requiring higher numbers of cells.
已开发出无血清培养条件，通过多能和巨噬细胞集落刺激因子的作用，从大鼠骨髓前体细胞的非贴壁部分分化出树突状细胞，这些因子还进一步补充了亚油酸、维生素E和维生素D3。树突状细胞刺激自体T细胞增殖的高效力证明了其辅助活性，而此类细胞对Fc受体依赖性吞噬作用呈阴性，这是巨噬细胞的一个特征。虽然树突状细胞对α-萘丁酸酯酶呈弱阳性，但它们强烈表达RT.1 II类抗原。显然，这些细胞代表了一种更分化的表型，因为它们表达核A/C型核纤层蛋白。通过向培养物中添加血清，树突状细胞发育成巨噬细胞，这些巨噬细胞对核纤层蛋白A/C呈阳性，对α-萘丁酸酯酶也呈强阳性。因此，这些树突状细胞属于髓系谱系，似乎血清因子在成熟水平上控制分化。还可以为树突状细胞的大规模培养建立合适的条件，这对于需要大量细胞的应用将是有用的。
