Thys Roberta C S, Westfahl Harry, Noreña Caciano P Z, Marczak Lígia D F, Silveira Nádya P, Cardoso Mateus B
Programa de Pos-Graduacao em Engenharia Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Biomacromolecules. 2008 Jul;9(7):1894-901. doi: 10.1021/bm800143w. Epub 2008 Jun 3.
The effect of alkaline treatment on the ultrastructure of C-type starch granules was investigated during the alkaline extraction of Araucaria angustifolia (pinhao) starch. The efficiency in protein removal was evaluated using intrinsic fluorescence and Kjeldahl's method. In parallel, morphological changes of starch granules were observed using scanning electron microscopy and atomic force microscopy. The starch crystallinity was monitored by wide-angle X-ray scattering and the lamellar structure was studied by small-angle X-ray scattering (SAXS). The paracrystalline model was employed to interpret the SAXS curves. It was found that the granular organization was significantly altered when alkaline solutions were used during the extraction. A partial degradation of B-type allomorph of starch and a significant compression of semicrystalline growth rings were observed.
在南洋杉淀粉的碱性提取过程中，研究了碱性处理对C型淀粉颗粒超微结构的影响。使用固有荧光和凯氏定氮法评估蛋白质去除效率。同时，利用扫描电子显微镜和原子力显微镜观察淀粉颗粒的形态变化。通过广角X射线散射监测淀粉结晶度，并通过小角X射线散射（SAXS）研究层状结构。采用准晶模型解释SAXS曲线。结果发现，在提取过程中使用碱性溶液时，颗粒组织发生了显著变化。观察到淀粉B型同质多晶型的部分降解以及半结晶生长环的显著压缩。
