Kowal Krzysztof, Zukowski Sebastian, Moniuszko Marcin, Bodzenta-Łukaszyk Anna
Department of Allergology and Internal Medicine, Medical University of Bialystok, Poland.
Folia Histochem Cytobiol. 2008;46(2):193-8. doi: 10.2478/v10042-008-0029-0.
Urokinase plasminogen activator (uPA) and its inhibitor (PAI-1) have been associated with asthma. The aim of this study was to evaluate concentration of uPA and PAI-1 in induced sputum of house dust mite allergic asthmatics (HDM-AAs). The study was performed on 19 HDM-AAs and 8 healthy nonatopic controls (HCs). Concentration of uPA and PAI-1 was evaluated in induced sputum supernatants using ELISA method. In HDM-AAs the median sputum concentration of uPA (128 pg/ml; 95% CI 99 to 183 pg/ml) and PAI-1 (4063 pg/ml; 95%CI 3319 to 4784 pg/ml) were significantly greater than in HCs (17 pg/ml; 95%CI 12 to 32 pg/ml; p<0.001 and 626 pg/ml; 95%CI 357 to 961 pg/ml; p<0.001 for uPA and PAI-1 respectively). The sputum concentration of uPA correlated with sputum total cell count (r=0.781; p=0.0001) and with logarithmically transformed exhaled nitric oxide concentration (eNO) (r=0.486; p=0.035) but not with FEV1 or bronchial reactivity to histamine. On the contrary, the sputum PAI-1 concentration correlated with FEV1 (r=-0,718; p=0.0005) and bronchial reactivity to histamine expressed as log(PC20) (r=-0.824; p<0.0001) but did not correlate with sputum total cell count or eNO. The results of this study support previous observations linking PAI-1 with airway remodeling and uPA with cellular inflammation. Moreover, the observed effect of uPA seems to be independent of its fibrynolytic activity.
尿激酶型纤溶酶原激活剂（uPA）及其抑制剂（PAI - 1）与哮喘有关。本研究的目的是评估屋尘螨过敏性哮喘患者（HDM - AAs）诱导痰中uPA和PAI - 1的浓度。该研究对19名HDM - AAs患者和8名健康非特应性对照者（HCs）进行。使用酶联免疫吸附测定（ELISA）方法评估诱导痰上清液中uPA和PAI - 1的浓度。在HDM - AAs患者中，uPA的痰中位浓度（128 pg/ml；95%置信区间99至183 pg/ml）和PAI - 1的痰中位浓度（4063 pg/ml；95%置信区间3319至4784 pg/ml）显著高于HCs（分别为17 pg/ml；95%置信区间12至32 pg/ml；p<0.001和626 pg/ml；95%置信区间357至961 pg/ml；uPA和PAI - 1的p均<0.001）。痰中uPA浓度与痰总细胞计数（r = 0.781；p = 0.0001）以及对数转换后的呼出一氧化氮浓度（eNO）（r = 0.486；p = 0.035）相关，但与第一秒用力呼气容积（FEV1）或对组胺的支气管反应性无关。相反，痰中PAI - 1浓度与FEV1（r = -0.718；p = 0.0005）以及以log(PC20)表示的对组胺的支气管反应性（r = -0.824；p<0.0001）相关，但与痰总细胞计数或eNO无关。本研究结果支持先前将PAI - 1与气道重塑以及uPA与细胞炎症联系起来的观察结果。此外，观察到的uPA的作用似乎与其纤维蛋白溶解活性无关。
