El-Kosasy Amira Mabrouk, Salem Maissa Yacoub, El-Bardicy Mohamed Galal, El-Rahman Mohamed Khaled Abd
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Chem Pharm Bull (Tokyo). 2008 Jun;56(6):753-7. doi: 10.1248/cpb.56.753.
Novel miniaturized polyvinyl chloride (PVC) membrane sensors in all-solid state graphite and platinum wire supports were developed, electrochemically evaluated and used for the assay of rivastigmine hydrogen tartrate drug (RIV). The RIV sensors are based on the formation of an ion-association complex between the drug cation and tetrakis(4-chlorophenyl)borate (TpClPB) anionic exchanger as electroactive material dispersed in a PVC matrix. Linear responses of 10(-2) - 10(-5) M and 10(-2) - 10(-4) M with cationic slopes of 56.4 mV and 53.6 mV over the pH range 4 - 7 were obtained by using the RIV-coated graphite (sensor 1) and platinum wire (sensor 2) membrane sensors, respectively. The proposed method displays useful analytical characteristics for the determination of RIV in Exelon capsules with average recoveries of 100.01+/-0.835, 100.09+/-0.896, and in plasma with average recoveries of 99.47+/-0.97, 99.58+/-0.82, and in rat brain homogenate with average recoveries of 98.16+/-1.62, 99.02+/-1.57, for sensors 1 and 2, respectively. The methods were also used to determine the intact drug in the presence of its degradation product and thus could be used as stability indicating methods. The results obtained by the proposed procedures were statistically analyzed and compared with those obtained by using a reported method. No significant difference for both accuracy and precision was observed.
开发了基于全固态石墨和铂丝支撑的新型小型聚氯乙烯（PVC）膜传感器，对其进行了电化学评估，并用于测定酒石酸利斯的明药物（RIV）。RIV传感器基于药物阳离子与四（4-氯苯基）硼酸酯（TpClPB）阴离子交换剂之间形成的离子缔合络合物，该阴离子交换剂作为电活性材料分散在PVC基质中。使用涂有RIV的石墨（传感器1）和铂丝（传感器2）膜传感器，分别在4 - 7的pH范围内获得了10（-2） - 10（-5）M和10（-2） - 10（-4）M的线性响应，阳离子斜率分别为56.4 mV和53.6 mV。所提出的方法对测定安理申胶囊中的RIV具有有用的分析特性，传感器1和传感器2的平均回收率分别为100.01±0.835、100.09±0.896，在血浆中的平均回收率分别为99.47±0.97、99.58±0.82，在大鼠脑匀浆中的平均回收率分别为98.16±1.62、99.02±1.57。该方法还用于在其降解产物存在的情况下测定完整药物，因此可用作稳定性指示方法。对所提出程序获得的结果进行了统计分析，并与使用报道方法获得的结果进行了比较。在准确性和精密度方面均未观察到显著差异。
