Eytan G D, Matheson M J, Racker E
J Biol Chem. 1976 Nov 10;251(21):6831-7.
Cytochrome oxidase, QH2-cytochrome c reductase, and the oligomycin-sensitive adenosine triphosphatase were incorporated into liposomes by a new procedure which yielded unidirectional orientation of the proteins. Cytochrome oxidase was reconstituted in the mitochrondrial orientation and the adenosine triphosphatase in the submitochondrial orientation. Reconstitutions were achieved by incubating the proteins at room temperature with liposomes which contained phosphatidylcholine, phosphatidylethanolamine, and an acidic phospholipid (cardiolipin, phosphatidylinositol, or phosphatidylserine). The incorporation occurred without added detergent or sonication. This incorporation procedure may serve as a model for the insertion of proteins in vivo.
细胞色素氧化酶、QH2-细胞色素c还原酶和寡霉素敏感的三磷酸腺苷酶通过一种新方法被整合到脂质体中，该方法使蛋白质实现单向定向排列。细胞色素氧化酶以线粒体方向重组，三磷酸腺苷酶以亚线粒体方向重组。通过将蛋白质与含有磷脂酰胆碱、磷脂酰乙醇胺和酸性磷脂（心磷脂、磷脂酰肌醇或磷脂酰丝氨酸）的脂质体在室温下孵育来实现重组。整合过程无需添加去污剂或超声处理。这种整合方法可作为体内蛋白质插入的模型。
