Yoshida Y, Yoshioka Y
Second Department of Physiology, Iwate Medical University.
No Shinkei Geka. 1991 May;19(5):421-7.
Proton nuclear magnetic resonance spectroscopy (1H-MRS) was employed in vitro to obtain profiles of various metabolites contained in some of the representative human brain tumors. Extracted samples from each normal and tumoral tissue were used to obtain high-resolution spectra. While N-acetylaspartate was unequivocally demonstrated in normal brain tissues, it could not be detected in brain tumors. Creatine was variably reduced in brain tumors relative to that in normal brain tissue, and was undetectable in neurinomas and pituitary adenomas. Choline was present in all tumors and appeared as relatively prominent peaks in the spectra for meningiomas, pituitary adenomas and metastatic brain tumors. In contrast, levels of creatine and inositol in these tumors were low. Neurinoma showed the largest inositol peak among the tumors examined. Thus compared to normal brain tissue, creatine, choline and inositol in tumors exhibited various degrees of characteristic changes. Therefore we attempted to categorize the spectral pattern of normal and tumoral tissues based on creatine/inositol and/or choline/inositol ratios. There were statistically significant differences between either creatine/inositol ratios and choline/inositol ratios in the tumor groups and the ratios found in normal brain tissue. These ratios also varied relative to different tumor groups. These data suggested that nuclear magnetic resonance spectroscopy might yield clinically useful information which can not be provided by already widely used magnetic resonance imaging and might be applicable to the differential diagnosis of some brain tumors.
采用体外质子核磁共振波谱法（1H-MRS）获取一些具有代表性的人脑肿瘤中所含各种代谢物的图谱。从每个正常组织和肿瘤组织中提取的样本用于获取高分辨率光谱。虽然在正常脑组织中明确检测到了N-乙酰天门冬氨酸，但在脑肿瘤中未检测到。与正常脑组织相比，脑肿瘤中的肌酸含量有不同程度的降低，在神经鞘瘤和垂体腺瘤中未检测到。胆碱存在于所有肿瘤中，在脑膜瘤、垂体腺瘤和脑转移瘤的光谱中表现为相对突出的峰。相比之下，这些肿瘤中的肌酸和肌醇水平较低。在所检查的肿瘤中，神经鞘瘤的肌醇峰最大。因此，与正常脑组织相比，肿瘤中的肌酸、胆碱和肌醇呈现出不同程度的特征性变化。因此，我们试图根据肌酸/肌醇和/或胆碱/肌醇比率对正常组织和肿瘤组织的光谱模式进行分类。肿瘤组的肌酸/肌醇比率和胆碱/肌醇比率与正常脑组织中的比率存在统计学上的显著差异。这些比率在不同肿瘤组之间也有所不同。这些数据表明，核磁共振波谱法可能产生临床上有用的信息，而这些信息是目前广泛使用的磁共振成像所无法提供的，并且可能适用于某些脑肿瘤的鉴别诊断。
