Gast R K, Beard C W
United States Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia 30605.
Poult Sci. 1991 May;70(5):1273-6. doi: 10.3382/ps.0701273.
Eggs laid by hens experimentally infected with Salmonella enteritidis were assayed for the presence of Serogroup D-specific yolk antibodies. Yolk antibodies were detected with S. enteritidis and Salmonella pullorum antigens in the microantiglobulin test as early as 9 days after inoculation of hens with S. enteritidis. Yolk antibody titers reached peak levels at 3 to 5 wk postinoculation and remained at detectable levels for at least 7 wk postinoculation in eggs from both orally inoculated and horizontally contact-exposed hens. Eggs laid by hens from commercial flocks implicated in epidemiological investigations of human S. enteritidis outbreaks were also tested. Serogroup D-specific yolk antibodies were detected in 5 to 22% of eggs from hens in houses identified as infected by bacteriological culturing of internal organs of hens for S. enteritidis.
对经实验感染肠炎沙门氏菌的母鸡所产鸡蛋进行检测，以确定是否存在D群特异性卵黄抗体。早在给母鸡接种肠炎沙门氏菌9天后，就在微抗球蛋白试验中用肠炎沙门氏菌和鸡白痢沙门氏菌抗原检测到了卵黄抗体。接种后3至5周，卵黄抗体滴度达到峰值水平，在经口服接种和水平接触暴露的母鸡所产鸡蛋中，接种后至少7周抗体滴度仍可检测到。对涉及人类肠炎沙门氏菌疫情流行病学调查的商业鸡群中的母鸡所产鸡蛋也进行了检测。在通过对母鸡内脏进行肠炎沙门氏菌细菌培养确定为感染鸡舍的母鸡所产鸡蛋中，5%至22%检测到了D群特异性卵黄抗体。
