Korf Ulrike, Henjes Frauke, Schmidt Christian, Tresch Achim, Mannsperger Heiko, Löbke Christian, Beissbarth Tim, Poustka Annemarie
Division of Molecular Genome Analysis, B050, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120, Heidelberg, Germany.
Adv Biochem Eng Biotechnol. 2008;110:153-75. doi: 10.1007/10_2008_101.
A significant bottleneck for the time-resolved and quantitative description of signaling networks is the limited sample capacity and sensitivity of existing methods. Recently, antibody microarrays have emerged as a promising experimental platform for the quantitative and comprehensive determination of protein abundance and protein phosphorylation. This review summarizes the development of microarray applications involving antibody-based capture of target proteins with a focus on quantitative applications. Technical aspects regarding the production of antibody microarrays, identification of suitable detection and capture antibody pairs, signal detection methods, detection limit, and data analysis are discussed in detail.
信号网络的时间分辨和定量描述的一个重大瓶颈是现有方法的样本容量和灵敏度有限。最近，抗体微阵列已成为一种有前景的实验平台，用于蛋白质丰度和蛋白质磷酸化的定量和全面测定。本综述总结了涉及基于抗体捕获靶蛋白的微阵列应用的发展，重点是定量应用。详细讨论了抗体微阵列生产、合适的检测和捕获抗体对的鉴定、信号检测方法、检测限和数据分析等技术方面。
