The structure of vocal sounds produced with the mouth closed or with the mouth open in treefrogs.

Frogs and toads mostly call with their mouths shut, unlike many other vertebrates. Sound is generated when air crosses the larynx, but there is no direct airflow to the external environment and radiation occurs at the skin. This study directly compares the acoustic output obtained from euthanized frogs with the mouth open against the output obtained with the mouth closed during activation of the larynx by airflow. With the mouth closed, the vocal sac was inflated and the acoustic energy was concentrated in the same harmonics as in the advertisement call, whereas with the mouth open, energy was spread in a wide range of harmonics. The acoustic output at the dominant frequency was more intense with the mouth closed than with the mouth open. More sound was radiated through the vocal sac and head than through the rest of the body. The spectral differences between open and closed mouth treatments matched the differences observed between natural advertisement calls, produced with the mouth closed, and distress calls, produced with the mouth open. By calling with the mouth closed, treefrogs can potentially produce advertisement calls with the energy concentrated in a narrower frequency range than with the mouth open.