Leão J C, Geracitano L A, Monserrat J M, Amado L L, Yunes J S
Unidade de Pesquisa em Cianobactérias, Fundação Universidade Federal do Rio Grande, Pós-Graduação em Oceanografia Biológica, Rio Grande/RS, Brazil.
Mar Environ Res. 2008 Jul;66(1):92-4. doi: 10.1016/j.marenvres.2008.02.033. Epub 2008 Feb 26.
Oxidative stress induced by microcystins was evaluated in an estuarine worm, Laeonereis acuta (Nereididae). Ten organisms were exposed to lyophilized cells of a toxic Microcystisaeruginosa strain RST9501 ( approximately 2 microg/mL microcystins, MC); 10 were exposed to lyophilized cells of a nontoxic Aphanotece sp. strain RSMan92 and 10 were maintained without cyanobacterial cells. Exposure time was 48 h. The enzymatic antioxidant defenses, as well as the oxidative damage, were analyzed. Toxic and nontoxic cyanobacteria lowered catalase activity with no changes in glutathione reductase and glutathione-S-transferase activities. This may have led to toxin intracellular accumulation, which should favor oxidative stress generation, observed by the high lipid peroxide and DNA-protein crosslink levels in the group exposed to MC.
在一种河口蠕虫锐足拉氏沙蚕（沙蚕科）中评估了微囊藻毒素诱导的氧化应激。将10只生物暴露于有毒铜绿微囊藻菌株RST9501的冻干细胞（约2微克/毫升微囊藻毒素，MC）；10只暴露于无毒隐球藻属菌株RSMan92的冻干细胞，10只在没有蓝藻细胞的情况下饲养。暴露时间为48小时。分析了酶促抗氧化防御以及氧化损伤。有毒和无毒蓝藻均降低了过氧化氢酶活性，而谷胱甘肽还原酶和谷胱甘肽-S-转移酶活性没有变化。这可能导致毒素在细胞内积累，这应该有利于氧化应激的产生，这在暴露于MC的组中通过高脂质过氧化物和DNA-蛋白质交联水平观察到。
