Lu Ya-Wei, Shen Wen-Tao, Tang Qing-Jie, Niu Yan-Mei, Zhou Peng
State Key Biotechnology Laboratory for Tropical Crops, Institute of BioScience and Biotechnology, CATAS, Haikou 571101, China.
Bing Du Xue Bao. 2008 Jun;24(2):152-4.
Total RNA was extracted from infected papaya (Carica papaya L.) leaves in Hainan Province, and the full-length sequences of papaya ringspot virus were amplified by RT-PCR and RACE, and its complete genomic sequence was assembled, named Hainan-P isolate. The RNA genome sequence of Hainan-P isolate was 10323 nucleotides (nts)in length,excluding the 3'-terminal poly(A) tail. And it was composed of a single open reading frame encoding a polyprotein of 3343 amino acids.. The result of homology analysis with twelve GenBank PRSV isolates showed that the polyprotein identity of Hainan-P ranged from 89. 8% to 93.2%, that was higher than the complete nt homology of 82.3% to 89.1%. The P1 amino acid was the least conserved (sharing homology only between 65.4% and 80.1%), whereas HC-Pro, CI and CP were the most conserved. Phylogenetic tree were constructed by the Neighbor-joining method in MEGA 3.1, which showed that PRSV isolates were obviously relevant to geographical origin, and it was impossible to delineate host-specific (P type and W type)evolution.
从海南省感染番木瓜（Carica papaya L.）的叶片中提取总RNA，通过RT-PCR和RACE扩增番木瓜环斑病毒的全长序列，并组装其完整基因组序列，命名为海南-P分离株。海南-P分离株的RNA基因组序列长度为10323个核苷酸（nts），不包括3'-末端的poly（A）尾。它由一个单一的开放阅读框组成，编码一个3343个氨基酸的多聚蛋白。与12个GenBank PRSV分离株的同源性分析结果表明，海南-P的多聚蛋白同一性在89.8%至93.2%之间，高于82.3%至89.1%的完整核苷酸同源性。P1氨基酸的保守性最低（同源性仅在65.4%至80.1%之间），而HC-Pro、CI和CP的保守性最高。利用MEGA 3.1中的邻接法构建系统发育树，结果表明PRSV分离株与地理来源明显相关，无法区分宿主特异性（P型和W型）进化。
